Strain information
 NBRP Rat No: 0657  Strain name: W-Tg(Per1-luc)Ylab  Commmon Name: Per1-lucWistar Rat Genome Database
Principal Investigator:  Shin Yamazaki   Vanderbilt University        Room U8231A, MRBIII, 465 21st Ave South     37232 Nashsville, TN      USA
Tel: USA (615)-322-8054     Fax: USA (615) 343-6707 Email: shin.yamazaki@vanderbilt.edu
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  Albino
Inbred Generations  Crl:WI (stock#003, CharlesRiver USA), N16 (May 10/2011)
Usage Restrictions  1:Use of this biological resource in the commercial enterprises is not permitted in principle.
2:Use of this biological resource by Companies or for-profit entities requires a license from University of Virginia Patent Foundation prior to shipping.

CONTACT FOR LICENSE INQUIRIES:
Stephanie A. Miller, Ph.D.
Licensing Associate
UVA Patent Foundation
250 W. Main St. Suite 300
Charlottesville, VA 22902
Phone: 434-982-1608
Email: stephanie@uvapf.org
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others Circadian Biology
 Control Strain  Marker Strain
Gene Affected mouse Period1 プロモーターが、Luciferase 発光レポーターを駆動する外来遺伝子をもつトランスジェニックラット
Origin The original work using this transgenic rat was published in Science (Yamazaki et al. 2000). This transgenic rat was generated in the Wistar outbred strain (Charles River, Japan). We maintained the L1 line. The transgenic rat was generated using a DNA construct in which the mouse Period1 promoter drives firefly luciferase (Hida et al, 2000).
Strain characteristics The circadian rhythm in Per1 transcription is easily monitored from tissue explants (Yamazaki and Takahashi 2005).
Breeding Conditions Good breeding performance, similar to the Wistar strain.
Genotyping Measuring luminescence from a tail snip is the best, most reliable, and easiest way to determine genotype. Aliquot ~20μl of a solution containing 0.1mM to 1mM beetle luciferin in water into eppendorf tubes. Measure the photon counts in tube containing only the luciferin solution as a black (usually around 50 cps). Place a freshly cut tail tip in the eppendorf tube (cut end submerged in solution) and measure the photon counts (over 10,000 cps). PCR genotype can also be performed using the following conditions: The transgene can be identified by PCR using the two primer pairs within the luciferase gene. PCR was performed at 95 °C (30 sec) for 1cycle, 95 °C (30 sec), 57 °C (30 sec),　72 °C (60 sec) for 30 cycles and 72 °C (60 sec) for 1 cycle. Primer sequences are: Luc F:TTTATAATGAACGTGAATTGCTC (323-345 of luciferase ORF) and Luc R:CGTATTTGTCAATCAGAGTGC (893-913of luciferase ORF ) . The size of PCR product is 590bp.
References
Hida A, Koike N, Hirose M, Hattori M, Sakaki Y, Tei H (2000) The human and mouse Period1 genes: five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription. Genomics 65:224-233.


Yamazaki S, Takahashi JS (2005) Real-time luminescence reporting of circadian gene expression in mammals. Methods Enzymol 393: 288-301.


Yamazaki S, Numano R, Abe M, Hida A, Takahashi RI, Ueda M, Block GD, Sakaki Y, Menaker M and Tei H (2000) Resetting central and peripheral circadian oscillators in transgenic rats. Science 288: 682-685.

Stokkan K-A, Yamazaki S, Tei H, Sakaki Y and Menaker M (2001) Entrainment of the circadian clock in the liver by feeding. Science 291: 490-493.

Abe M, Herzog ED, Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Circadian rhythms in isolated brain regions. J Neurosci 22: 356-356.

Davidson AJ, Stokkan K-A, Yamazaki S, Menaker M (2002) Food-anticipatory activity and liver Per1-luciferase expression in diabetic transgenic rats. Physiol Behav 76: 21-26.

Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Effects of aging on central and peripheral mammalian clocks. Proc Natl Acad Sci USA 99: 10801-10806.

Vansteensel MJ, Yamazaki S, Albus H, Deboer T, Block GD, Meijer JH, (2003) Dissociation between circadian Per1, neuronal and behavioral rhythms following a shifted environmental cycle. Curr Biol 13:1538-1542

Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M (2009) Ontogeny of Circadian Organization in the Rat. J Biol Rhythms 24:55-63.

Stokkan K-A, Yamazaki S, Tei H, Sakaki Y and Menaker M (2001) Entrainment of the circadian clock in the liver by feeding. Science 291: 490-493.

Abe M, Herzog ED, Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Circadian rhythms in isolated brain regions. J Neurosci 22: 356-356.



Davidson AJ, Stokkan K-A, Yamazaki S, Menaker M (2002) Food-anticipatory activity and liver Per1-luciferase expression in diabetic transgenic rats. Physiol Behav 76: 21-26.

Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Effects of aging on central and peripheral mammalian clocks. Proc Natl Acad Sci USA 99: 10801-10806

Vansteensel MJ, Yamazaki S, Albus H, Deboer T, Block GD, Meijer JH, (2003) Dissociation between circadian Per1, neuronal and behavioral rhythms following a shifted environmental cycle. Curr Biol 13:1538-1542

Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M (2009) Ontogeny of Circadian Organization in the Rat. J Biol Rhythms 24:55-63.
Additional strain information