Strain information
 NBRP Rat No: 0639  Strain name: SD-Tg(Gfap-Tk)Jog  Commmon Name: GFAP-TK Rat Genome Database
Principal Investigator:  Jonathan flint   University of Oxford        Roosevelt Drive      OX3 7BN Oxford      UK
Tel: +44 (0)1865 287772     Fax: +44 (0)1865 287501 Email:  jf@well.ox.ac.uk
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  White
Inbred Generations  More than 10
Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Tk: thymidine kinase Gfap: glial fibrillary acidic protein
Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA). The GFAP-TK rat was generated by pronuclear injection of a‘Gfap-Tk' Bacterial Artificial Chromosome (BAC) construct, engineered using ‘RedET’-mediated homologous recombination methods (Groves et al., 2013).
Strain characteristics Rats show no phenotype until dosed with the antiviral agant ganciclovir. Chronic dosing at 10mg/kg/day via subcutaneous minipump for 21 days results in a loss of DCX-positive cells in the dentate gyrus and subventricular zone.
Breeding Conditions Males are infertile. Females generate good litters of 10-15 pups showing 50% heterozygous offspring.
Genotyping GFAP-TK transgene can be identified using the foolwoing primers and protocols: GFAP-TK_genoF cca acg gcg acc tgt ata ac GFAP-TK_genoR gcc ctt tag tga ggg tta att c Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever
References Groves JO, Leslie I, Huang GJ, McHugh SB, Taylor A, Mott R, Munafò M, Bannerman DM, Flint J.
Ablating Adult Neurogenesis in the Rat Has No Effect on Spatial Processing: Evidence from a Novel Pharmacogenetic Model.
PLoS Genet. 2013;9(9):e1003718. doi: 10.1371/journal.pgen.1003718. Epub 2013 Sep 5.
Additional strain information