Strain information
 NBRP Rat No: 0640  Strain name: SD-Tg(Dsp-mCherry,-DTR)Jog  Commmon Name: DSP-STOP-hDTR Rat Genome Database
Principal Investigator:  Jonathan flint   University of Oxford        Roosevelt Drive      OX3 7BN Oxford      UK
Tel: +44 (0)1865 287772     Fax: +44 (0)1865 287501 Email:  jf@well.ox.ac.uk
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  White
Inbred Generations  More than 5
Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Genetically Modified (GM)  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Dsp
Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA).
Strain characteristics This rat carries a transgene consisting of the Dsp promoter followed by a 'STOP’ cassette flanked by FRT sites. The stop cassette consists of an mcherry gene encoding for a red fluorescent protein and a kanamycin resistant gene and three SV40polyA adenylation signals. The STOP cassette is followed by a gene encoding for the human diphtheria toxin receptor. Red fluorescence can be detected from the skin of the animals but not the brain.
Breeding Conditions Both males and fertiles are fertile and generate good litter sizes of 10-15 pups.
Genotyping The transgene can be detected using the following primers and PCR protocol: DSP+DTR_gno2_F cca agc tga agg tga cca ag DSP+DTR_gno2_R tgg tgt agt cct cgt tgt gg Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever
References
Additional strain information