Strain information  NBRP Rat No: 0640  Strain name: SD-Tg(Dsp-mCherry,-DTR)Jog  Commmon Name: DSP-STOP-hDTR Rat Genome Database Principal Investigator:  Jonathan flint   University of Oxford        Roosevelt Drive      OX3 7BN Oxford      UK Tel: +44 (0)1865 287772     Fax: +44 (0)1865 287501 Email:  jf@well.ox.ac.uk Preservation Status:   Embryo        Sperm       Living Animals Coat Color  White Inbred Generations  More than 5 Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Dsp Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA). Strain characteristics This rat carries a transgene consisting of the Dsp promoter followed by a 'STOP’ cassette flanked by FRT sites. The stop cassette consists of an mcherry gene encoding for a red fluorescent protein and a kanamycin resistant gene and three SV40polyA adenylation signals. The STOP cassette is followed by a gene encoding for the human diphtheria toxin receptor. Red fluorescence can be detected from the skin of the animals but not the brain. Breeding Conditions Both males and fertiles are fertile and generate good litter sizes of 10-15 pups. Genotyping The transgene can be detected using the following primers and PCR protocol: DSP+DTR_gno2_F cca agc tga agg tga cca ag DSP+DTR_gno2_R tgg tgt agt cct cgt tgt gg Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever References Additional strain information