Strain information
 NBRP Rat No: 0641  Strain name: SD-Tg(Neurod6-mCherry,-DTR)Jog  Commmon Name: NeuroD6-stop-hDTR Rat Genome Database
Principal Investigator:  Jonathan flint   University of Oxford        Roosevelt Drive      OX3 7BN Oxford      UK
Tel: +44 (0)1865 287772     Fax: +44 (0)1865 287501 Email:  jf@well.ox.ac.uk
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  
Inbred Generations  F2
Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected NeuroD6
Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA).
Strain characteristics This rat carries a transgene consisting of the NeuroD6 promoter followed by a 'STOP’ cassette flanked by FRT sites. The stop cassette consists of an mcherry gene encoding for a red fluorescent protein and a kanamycin resistant gene and three SV40polyA adenylation signals. The STOP cassette is followed by a gene encoding for the human diphtheria toxin receptor. This model is yet to be validated.
Breeding Conditions As yet unknown.
Genotyping The following primers and protocol can be used to detect the presence of the NeuroD6-stop-hDTR transgene: neo_F AGG ATC TCC TGT CAT CTC ACC TTG CTC CTG neo_R AAG AAC TCG TCA AGA AGG CGA TAG AAG GCG Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever
References
Additional strain information