Strain information  NBRP Rat No: 0642  Strain name: SD-Tg(Camk2a-FLPo)Jog  Commmon Name: CamK2a-flpO Rat Genome Database Principal Investigator:  Jonathan flint   University of Oxford        OX3 7BN Roosevelt Drive     Oxford     UK Tel: 44 (0)1865 287772    Fax: 44 (0)1865 287501 Email: jf@well.ox.ac.uk Preservation Status:   Embryo        Sperm       Living Animals Coat Color  white Inbred Generations  More than 10 Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected CAMK2A Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA). The detailed FLPo expression pattern has not yet been analyzed. (Jun, 2017) Strain characteristics This transgene consists of approximately 6kb of the CamK2a promoter followed by a gene encoding FLP, optimised for mamalian exprssion (FLPo). This rat remains unvalidated. The detailed expression pattern of FLPo has not yet been examined. Breeding Conditions Both males and females are fertile and generate good litters sizes of 10-15 pups at 50% heterozygosity when bred with WT mates. Genotyping The CamK2a-FLPo transgene can be detected usingthe follwoign primers and PCR protocol: CamK_flp_F aga atg tgg cac cca cta gc CamK_flp_R tct tct tgc tgt ggc tgt tg Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever References Additional strain information