Strain information
NBRP Rat No: 0648
Strain name
: TM-
Prkdc
em4Kyo
Il2rg
em5Kyo
Commmon Name: TM-DSCID
Rat Genome Database
Principal Investigator:
Tomoji Mashimo The University of Tokyo, The Institute of Medical Science 4-6-1 Shirokanedai-Minatoku-Tokyoto 108-8639 Japan
Tel: 03-6409-2489 Fax:
Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
albino (c)
Inbred Generations
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Prkdc: protein kinase, DNA activated, catalytic polypeptide, Il2rg: interleukin 2 receptor-gamma
Origin
This strain was established by Zinc-finger nucleases (ZFNs) method taregting rat Prkdc gene and Il2rg gene. Background strain: TM/Kyo
Strain characteristics
This strain shows severe combined immunodeficiency(SCID)and growth retardation caused by a 20-bp deletion in the exon 1 of Prkdc gene and a 653-bp deletion in Il2rg gene.
Breeding Conditions
maintaied by mating between Il2rg-homozygous/Prkdc-heterozygous rats
Genotyping
Prkdc wild:309-bp, mutant:289-bp (primers:CTTGCGGTGCTGGCTACTAC、TTCCCTCTTGTACTCTCATCCAA) Il2rg wild:1509-bp, mutant:856-bp (primers: TCTCCCAGGGGACTTAGCTTA、TGGGACCTGGTGTTAGGTTT)
References
Additional strain information