Rat Strains

Principal Investigator:

Hiromu Yawo Tohoku University 2-1-1, Katahira, Aoba-ku, Sendai-shi 980-8577 Miyagi Japan

Preservation Status:

Embryo Sperm Living Animals

Coat Color

白

Inbred Generations

10

Usage Restrictions

Any form of publications of data resulting from the use of the BIOROGICAL RESOURCE should reference the following DEPOSITOR’s papers.
“Tomita, H., Sugano, E., Fukazawa, Y., Isago, H., Sugiyama, Y., Hiroi, T., Ishizuka, T., Mushiake, H., Kato, M., Hirabayashi, M., Shigemoto, R., Yawo, H. and Tamai, M. Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS ONE 4(11), e7679 (2009)”
In publishing, an acknowledgment to the DEPOSITOR is requested.
The BIOROGICAL RESOURCE should be used under the following rules.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOUCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University.

Genetic Status

Comercial Availability

Research Category

Gene Affected

オワンクラゲGFPの改変体、チャネルロドプシン2(ChR2)

Origin

The transgenic rats were established by injection of transgene consisting of ChR2 and Venus fused transgene driven by the Thy-1.2 promoter into Wistar rat fertile eggs.

Strain characteristics

Thy-1.2 activated cells in these strains can express ChR2-Venus fused protein and seem to have a sensitibity to the light.

Breeding Conditions

Good breeding performance.

Genotyping

(1) PCR: detection of 720 bp band for Venus. Primers are Venus-F: 5’-atggtgagcaagggcgaggagctgt-3’ (25-mer), Venus-R: 5’-ttacttgtacagctcgtccatgccga-3’ (26-mer). (2) fluorescence detection using fresh tail sample: Venus fluorescence can be detected in myelinated nerve fiber.

References

Daichi Sueto, Eriko I, Akihisa Onishi, Makoto Tsuda
Spinal dorsal horn neurons involved in the alleviating effects of cannabinoid receptor agonists on neuropathic allodynia-like behaviors in rats
J Pharmacol Sci. 2025 Apr;157(4):253-260.

Sueto D, Onishi A, I E, Yoshikawa Y, Tsuda M.
Laminar-selective spinal astrocyte population capable of converting tactile information into nociceptive in rats.
J Pharmacol Sci. 2024 Apr;154(4):312-315.

Ishibashi T, Yoshikawa Y, Sueto D, Tashima R, Tozaki-Saitoh H, Koga K, Yamaura K, Tsuda M.
Selective Involvement of a Subset of Spinal Dorsal Horn Neurons Operated by a Prodynorphin Promoter in Aβ Fiber-Mediated Neuropathic Allodynia-Like Behavioral Responses in Rats.
Front Mol Neurosci. 2022 Jun 23:15:911122.

Tashima R, Koga K, Yoshikawa Y, Sekine M, Watanabe M, Tozaki-Saitoh H, Furue H, Yasaka T, Tsuda M.
A subset of spinal dorsal horn interneurons crucial for gating touch-evoked pain-like behavior.
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2021220118.

Ohmichi Y, Ohmichi M, Tashima R, Osuka K, Fukushige K, Kanikowska D, Fukazawa Y, Yawo H, Tsuda M, Naito M, Nakano T.
Physical disuse contributes to widespread chronic mechanical hyperalgesia, tactile allodynia, and cold allodynia through neurogenic inflammation and spino-parabrachio-amygdaloid pathway activation.
Pain. 2020 Aug;161(8):1808-1823.

Michael Shur, Florian Fallegger, Elvira Pirondini, Adrien Roux, Arnaud Bichat, Quentin Barraud, Gregoire Courtine, and Stephanie P. Lacour
Soft Printable Electrode Coating for Neural Interfaces
ACS Appl Bio Mater. 2020 Jul 20;3(7):4388-4397.

Liu Y, Ohshiro T, Sakuragi S, Koizumi K, Mushiake H, Ishizuka T, Yawo H.
Optogenetic study of the response interaction among multi-afferent inputs in the barrel cortex of rats.
Sci Rep. 2019 Mar 8;9(1):3917.

Abe K, Yawo H.
Quantitative study of the somatosensory sensitization underlying cross-modal plasticity.
PLoS One. 2018 Dec 5;13(12):e0208089.

Tashima R, Koga K, Sekine M, Kanehisa K, Kohro Y, Tominaga K, Matsushita K, Tozaki-Saitoh H, Fukazawa Y, Inoue K, Yawo H, Furue H, Tsuda M.
Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-like Sensory and Emotional Behaviors after Nerve Injury in Rats.
eNeuro. 2018 Feb 15;5(1). pii: ENEURO.0450-17.2018.

Abe K, Yawo H.
Optogenetic conditioning of paradigm and pattern discrimination in the rat somatosensory system.
PLoS One. 2017 Dec 21;12(12):e0189439.

Lee W, Kim D, Matsuhisa N, Nagase M, Sekino M, Malliaras GG, Yokota T, Someya T.
Transparent, conformable, active multielectrode array using organic electrochemical transistors.
Proc Natl Acad Sci U S A. 2017 Sep 18. pii: 201703886

Jimbo Y, Matsuhisa N, Lee W, Zalar P, Jinno H, Yokota T, Sekino M, Someya T.
Ultraflexible Transparent Oxide/Metal/Oxide Stack Electrode with Low Sheet Resistance for Electrophysiological Measurements.
ACS Appl Mater Interfaces. 2017 Oct 11;9(40):34744-34750.

Lee W, Kim D, Rivnay J, Matsuhisa N, Lonjaret T, Yokota T, Yawo H, Sekino M, Malliaras G. G and Someya T.
Integration of Organic Electrochemical and Field-Effect Transistors for Ultra flexible, High Temporal Resolution Electrophysiology Arrays.
Adv. Mater. 2016, DOI: 10.1002/adma.201602237

Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M (2009) Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS One. Nov 5;4(11):e7679.

Ji Z-G, Ito S, Honjoh T, Ohta H, Ishizuka T, Fukazawa Y, Yawo H (2012) Light-evoked somatosensory perception of transgenic rats which express channelrhodopsin-2 in dorsal root ganglion cells. PLoS ONE 7:e32699.

Additional strain information