Strain information
 NBRP Rat No: 0686  Strain name: W-Tg(Thy1-COP4/YFP*)5Jfhy  Commmon Name: W-TChR2V5, W-Tg(Thy1.2-ChR2Venus)5Jfhy Rat Genome Database
Principal Investigator:  Hiromu Yawo  Tohoku University       2-1-1, Katahira, Aoba-ku, Sendai-shi    980-8577 Miyagi     Japan
Tel: 022-217-6208    Fax: 022-217-6211 Email: hiromu.yawo.c7@tohoku.ac.jp （担当　五十嵐 敬幸：hirobio@med.tohoku.ac.jp）
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白
Inbred Generations  3
Usage Restrictions  Any form of publications of data resulting from the use of the BIOROGICAL RESOURCE should reference the following DEPOSITOR’s papers.
“Tomita, H., Sugano, E., Fukazawa, Y., Isago, H., Sugiyama, Y., Hiroi, T., Ishizuka, T., Mushiake, H., Kato, M., Hirabayashi, M., Shigemoto, R., Yawo, H. and Tamai, M. Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS ONE 4(11), e7679 (2009)”
In publishing, an acknowledgment to the DEPOSITOR is requested.
The BIOROGICAL RESOURCE should be used under the following rules.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOUCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected オワンクラゲGFPの改変体、チャネルロドプシン2(ChR2)
Origin The transgenic rats were established by injection of transgene consisting of ChR2 and Venus fused transgene driven by the Thy-1.2 promoter into Wistar rat fertile eggs.
Strain characteristics Thy-1.2 activated cells in these strains can express ChR2-Venus fused protein and seem to have a sensitibity to the light.
Breeding Conditions Good breeding performance.
Genotyping (1) PCR: detection of 720 bp band for Venus. Primers are Venus-F: 5’-atggtgagcaagggcgaggagctgt-3’ (25-mer), Venus-R: 5’-ttacttgtacagctcgtccatgccga-3’ (26-mer). (2) fluorescence detection using fresh tail sample: Venus fluorescence can be detected in myelinated nerve fiber.
References Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M (2009) Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS One. Nov 5;4(11):e7679.

Additional strain information