Rat Strains

Principal Investigator:

Shizuko Nagao Fujita Health University 1-98 Dengakugakubo, Toyoake-shi 470-1192 Aichi Japan

Preservation Status:

Embryo Sperm Living Animals

Coat Color

アルビノ

Inbred Generations

F12以上

Usage Restrictions

Recipient shall obtain a letter of acceptance from depositor for the use of BIOLOGICAL RESOURCE in advance.
Recipient also shall discuss the terms of collaboration with depositor.

Genetic Status

Comercial Availability

Research Category

Gene Affected

Anks6 (Pkdr1)

Origin

This strain rats was provided to University of Kansas from Central Institute for Laboratory Animal Breeding (Hanover, Germany), and then was introduced to Fujita Health University .

Strain characteristics

The causative gene of this rat is named as "Cy" or "Anks6 (Pkdr1)" (commonly expressed as "Cy"). This gene product is "SamCystin" . Cy is inherited in an autosomal dominant manner. The Cy/Cy homozygous and Cy/+ heterozygous rats develop polycystic kidney disease (PKD). The Cy/Cy homozygous rats die of renal failure at around 3 weeks of age. In the Cy/+ heterozygous rats, female rats survive for over 1 year, but the life-span of male rats is within 1 year. In the Cy/+ heterozygous kidneys, cell growth is promoted by activation of signaling pathway such as ERK, and the levels of MCP-1 and osteopontin are increased. In addition, cell growth is promoted by androgen receptor-mediated activation of signaling pathway in the heterozygous male rats.

Breeding Conditions

Mating between Cy/+ heterozygous rats.

Genotyping

Primers: 5’-CTAGAAGCCTCAGTGACCCC-3’and 5’-CAGCGTGTGAACAAGGTAGG-3’ PCR product (242 bp) is digested with MspI. After digestion, two bands (157 bp and 85 bp) are generated in the wild-type rats and only a single band (242 bp) is detected in the homozygous rats.

References

・Kaspareit-Rittinghausen J, Rapp K, Deerberg F, Wcislo A, Messow C. Hereditary polycystic kidney disease associated with osteorenal syndrome in rats. Vet Pathol. 1989 May;26(3):195-201.

・Cowley BD Jr, Gudapaty S, Kraybill AL, Barash BD, Harding MA, Calvet JP, Gattone VH 2nd. Autosomal-dominant polycystic kidney disease in the rat. Kidney Int. 1993 Mar;43(3):522-34.

・Brown JH, Bihoreau MT, Hoffmann S, Kränzlin B, Tychinskaya I, Obermüller N, Podlich D, Boehn SN, Kaisaki PJ, Megel N, Danoy P, Copley RR, Broxholme J, Witzgall R, Lathrop M, Gretz N, Gauguier D. Missense mutation in sterile alpha motif of novel protein SamCystin is associated with polycystic kidney disease in (cy/+) rat. J Am Soc Nephrol. 2005 Dec;16(12):3517-26. Epub 2005 Oct 5.

・長尾静子、釘田雅則、吉原大輔、山口太美雄　多発性嚢胞腎発症のメカニズム　Annual Review腎臓2013　中外医学社

・Nagao S, Kugita M, Yoshihara D, Yamaguchi T. Animal models for human polycystic kidney disease. Exp Anim. 2012;61(5):477-88.

・Nagao S, Yamaguchi T. PPAR-γ agonists in polycystic kidney disease with frequent development of cardiovascular disorders. Curr Mol Pharmacol. 2012 Jun;5(2):292-300.

・Toyohara T, Suzuki T, Akiyama Y, Yoshihara D, Takeuchi Y, Mishima E, Kikuchi K, Suzuki C, Tanemoto M, Ito S, Nagao S, Soga T, Abe T. Metabolomic profiling of the autosomal dominant polycystic kidney disease rat model. Clin Exp Nephrol. 2011 Oct;15(5):676-87. doi: 10.1007/s10157-011-0467-4. Epub 2011 Jun 22.

・Kugita M, Nishii K, Morita M, Yoshihara D, Kowa-Sugiyama H, Yamada K, Yamaguchi T, Wallace DP, Calvet JP, Kurahashi H, Nagao S. Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling. Am J Physiol Renal Physiol. 2011 Jan;300(1):F177-88.

・Nagao S, Morita M, Kugita M, Yoshihara D, Yamaguchi T, Kurahashi H, Calvet JP, Wallace DP. Polycystic kidney disease in Han:SPRD Cy rats is associated with elevated expression and mislocalization of SamCystin. Am J Physiol Renal Physiol. 2010 Nov;299(5):F1078-86.

・Nagao S, Nishii K, Yoshihara D, Kurahashi H, Nagaoka K, Yamashita T, Takahashi H, Yamaguchi T, Calvet JP, Wallace DP. Calcium channel inhibition accelerates polycystic kidney disease progression in the Cy/+ rat. Kidney Int. 2008 Feb;73(3):269-77. Epub 2007 Oct 17.

・Nagao S, Kusaka M, Nishii K, Marunouchi T, Kurahashi H, Takahashi H, Grantham J. Androgen receptor pathway in rats with autosomal dominant polycystic kidney disease. J Am Soc Nephrol. 2005 Jul;16(7):2052-62. Epub 2005 May 11.

・Nagao S, Yamaguchi T, Kusaka M, Maser RL, Takahashi H, Cowley BD, Grantham JJ. Renal activation of extracellular signal-regulated kinase in rats with autosomal-dominant polycystic kidney disease. Kidney Int. 2003 Feb;63(2):427-37.

・Kanno Y, Okada H, Moriwaki K, Nagao S, Takahashi H, Suzuki H. The effects of antihypertensive agents on the survival rate of polycystic kidney disease in Han:SPRD rats. Hypertens Res. 2002 Nov;25(6):939-43.

・Cowley BD Jr, Ricardo SD, Nagao S, Diamond JR. Increased renal expression of monocyte chemoattractant protein-1 and osteopontin in ADPKD in rats. Kidney Int. 2001 Dec;60(6):2087-96.

Additional strain information