Strain information
 NBRP Rat No: 0679  Strain name: DA.PVG.1AV1-(D17Rat8-D17Rat37)/Kini  Commmon Name: DA.PVG.1AV1-Eae23 Rat Genome Database
Principal Investigator:  Tomas Olsson  Karolinska Institutet       CMM, Karolinska University Hospital, 171 76,     L8:04, Stockholm     Sweden
Tel: +46 8 51776258    Fax: +46 8 51775562 Email: tomas.olsson@ki.se
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/ ../images/Photos/
Coat Color  Agouti
Inbred Generations  Congenic strain was establiesh using a “speed congenic” strategy in 2008. Since then, the strain was kept as inbred.
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in the following article:
Stridh, P Thessen Hedreul, M Beyeen, AD Adzemovic, MZ Laaksonen, H Gillett, A Ockinger, J Marta, M Lassmann, H Becanovic, K Jagodic, M Olsson, T. Fine-mapping resolves Eae23 into two QTLs and implicates ZEB1 as a candidate gene regulating experimental neuroinflammation in rat. PLos One, 2010 Sep 15;5(9):e12716. PMID:20856809.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected > 47 Mb
Origin Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997) with 8 microsatellite markers from the telomeric marker D17Rat8 to D17Rat37, and 95 microsatellite markers outside the congenic regions. In the N7 generation, all 95 background markers were fixed as DA homozygous. One further backcross was performed and chromosome 17 (Eae23) heterozygous rats subsequently intercrossed to produce the congenic strain DA.PVG.1AV1-(D17Rat8-D17Rat37) in 2008. Since then, the strain was kept as inbred.
Strain characteristics Partial protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain (no significant difference in susceptibility (INC and ONS), but DA.PVG-Eae23 rats had lower severity. CNS has increased level of Foxp3 positive cells.
Breeding Conditions Good breeding performance.
Genotyping Flanking markers inside the congenic fragment: D17Rat8 and D17Rat37. Flanking markers outside the congenic fragment: D17Rat6 and D17Rat172.
References Stridh, P Thessen Hedreul, M Beyeen, AD Adzemovic, MZ Laaksonen, H Gillett, A Ockinger, J Marta, M Lassmann, H Becanovic, K Jagodic, M Olsson, T. Fine-mapping resolves Eae23 into two QTLs and implicates ZEB1 as a candidate gene regulating experimental neuroinflammation in rat. PLoS One. 2010 Sep 15;5(9):e12716. PMID:20856809.
Additional strain information