| NBRP Rat No: 0682 |
Strain name: DA.PVG.1AV1-(D10Rat81-D10Rat238)/Kini |
Commmon Name: DA.PVG.1AV1-Eae18a |
Rat Genome Database |
| Principal Investigator: |
Tomas Olsson Karolinska Institutet CMM, Karolinska University Hospital, 171 76, L8:04, Stockholm Sweden |
| Tel: +46 8 51776258 Fax: +46 8 51775562 |
Email: tomas.olsson@ki.se |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
Agouti |
| Inbred Generations |
Congenic strain was establiesh from the 10th generation advanced intercross line after 10 generations of backcrossing in 2010. Since then, the strain was kept as inbred. |
| Usage Restrictions |
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. |
| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
> 13.0 Mb |
| Origin |
Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Animals for congenic breeding were selected from the 10th generation of an advanced intercross line (AIL) originating from the EAE-susceptible DA and EAE-resistant PVG.av1 rat strains. Selected males containing a PVG.av1 fragment in the Eae18a region (from D10Rat81 to D10Rat238 marker, at 49.62 Mb and 62.68 Mb, respectively) were backcrossed with DA females for 10 generations, and offspring were genotyped using microsatellite markers in each generation. In the 10th generation, heterozygous males and females were crossed to obtain the experimental population of homozygous congenic rats and littermate controls. |
| Strain characteristics |
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| Breeding Conditions |
Good breeding performance. |
| Genotyping |
Flanking markers inside the congenic fragment: D10Rat81-D10Rat238. |
| References |
Jagodic M, Becanovic K, Sheng JR, Wu X, Bäckdahl L, Lorentzen JC, Wallström E, Olsson T. An advanced intercross line resolves Eae18 into two narrow quantitative trait loci syntenic to multiple sclerosis candidate loci. J Immunol. 2004 Jul 15;173(2):1366-73. PMID: 15240732. Ockinger J, Serrano-Fernández P, Möller S, Ibrahim SM, Olsson T, Jagodic M. Definition of a 1.06-Mb region linked to neuroinflammation in humans, rats and mice. Genetics. 2006 Jul;173(3):1539-45. Epub 2006 Apr 19. PMID: 16624898. |
| Additional strain information |
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