NBRP Rat No: 0683 |
Strain name: DA.BN-(D9Wox24-D9Rat20)/Kini |
Commmon Name: DA.BN-Eae4 |
Rat Genome Database |
Principal Investigator: |
Tomas Olsson Karolinska Institutet CMM, Karolinska University Hospital, 171 76, L8:04, Stockholm Sweden |
Tel: +46 8 51776258 Fax: +46 8 51775562 |
Email: tomas.olsson@ki.se |
Preservation Status: |
Embryo Sperm Living Animals |
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Coat Color |
Agouti |
Inbred Generations |
Congenic strain was establiesh using a “speed congenic” strategy as N6F1 in 1999. Since then, the strain was kept as inbred. |
Usage Restrictions |
In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development and the characterization of the strain has been published in the floowing article: Jagodic M, Colacios C, Nohra R, Degean AS, Beyeen AD, Khademi M, Casemayou A, Llamouroux L, Duthoit C, Papapietro O, Sjoholm L, Bernard ILagrange D, Dahlman I, Lundmark F, Otural AB, Soendegaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV,Sadovnick DA, Ebers GC, Seddigzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Foumie GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple scierosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID: 20368159. |
Genetic Status |
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Comercial Availability |
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Research Category |
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Gene Affected |
> 50 Mb (positionally cloned to Vav1 gene using interval-specific congenic lines) |
Origin |
Inbred Dark Agouti (DA) and Brown Norway (BN) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997). Briefly, in each backcross generation, rats for further breeding were selected on the basis of genotyping of 8 microsatellite markers in the congenic region, from marker D9Wox24 to D9Rat20. The genetic background of rats was also screened with 100 markers. After the complete removal of the donor genome outside the congenic fragment, two heterozygous rats were intercrossed to produce the homozygous strains DA.BN-Eae4(N7F1). |
Strain characteristics |
Protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain, regulation of proinflammatory T cell activation and regulatory T cells. |
Breeding Conditions |
Good breeding performance. |
Genotyping |
Flanking markers inside the congenic fragment: D9Wox24-D9Rat20. |
References |
Dahlman I, Jacobsson L, Glaser A, Lorentzen JC, Andersson M, Luthman H, Olsson T. Genome-wide linkage analysis of chronic relapsing experimental autoimmune encephalomyelitis in the rat identifies a major susceptibility locus on chromosome 9. J Immunol. 1999 Mar 1;162(5):2581-8. PMID: 10072499. Jagodic M, Colacios C, Nohra R, Dejean AS, Beyeen AD, Khademi M, Casemayou A, Lamouroux L, Duthoit C, Papapietro O, Sjöholm L, Bernard I, Lagrange D, Dahlman I, Lundmark F, Oturai AB, Soendergaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV, Sadovnick DA, Ebers GC, Seddighzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Fournié GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple sclerosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID: 20368159. Colacios C, Casemayou A, Dejean AS, Gaits-Iacovoni F, Pedros C, Bernard I, Lagrange D, Deckert M, Lamouroux L, Jagodic M, Olsson T, Liblau RS, Fournié GJ, Saoudi A. The p.Arg63Trp polymorphism controls Vav1 functions and Foxp3 regulatory T cell development. J Exp Med. 2011 Oct 24;208(11):2183-91. Epub 2011 Sep 26. PMID: 21948080. |
Additional strain information |
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