Strain information
 NBRP Rat No: 0683  Strain name: DA.BN-(D9Wox24-D9Rat20)/Kini  Commmon Name: DA.BN-Eae4 Rat Genome Database
Principal Investigator:  Tomas Olsson  Karolinska Institutet       CMM, Karolinska University Hospital, 171 76,     L8:04, Stockholm     Sweden
Tel: +46 8 51776258    Fax: +46 8 51775562 Email: tomas.olsson@ki.se
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/ ../images/Photos/
Coat Color  Agouti
Inbred Generations  Congenic strain was establiesh using a “speed congenic” strategy as N6F1 in 1999. Since then, the strain was kept as inbred.
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in the floowing article:
Jagodic M, Colacios C, Nohra R, Degean AS, Beyeen AD, Khademi M, Casemayou A, Llamouroux L, Duthoit C, Papapietro O, Sjoholm L, Bernard ILagrange D, Dahlman I, Lundmark F, Otural AB, Soendegaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV,Sadovnick DA, Ebers GC, Seddigzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Foumie GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple scierosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID: 20368159.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected > 50 Mb (positionally cloned to Vav1 gene using interval-specific congenic lines)
Origin Inbred Dark Agouti (DA) and Brown Norway (BN) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997). Briefly, in each backcross generation, rats for further breeding were selected on the basis of genotyping of 8 microsatellite markers in the congenic region, from marker D9Wox24 to D9Rat20. The genetic background of rats was also screened with 100 markers. After the complete removal of the donor genome outside the congenic fragment, two heterozygous rats were intercrossed to produce the homozygous strains DA.BN-Eae4(N7F1).
Strain characteristics Protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain, regulation of proinflammatory T cell activation and regulatory T cells.
Breeding Conditions Good breeding performance.
Genotyping Flanking markers inside the congenic fragment: D9Wox24-D9Rat20.
References Dahlman I, Jacobsson L, Glaser A, Lorentzen JC, Andersson M, Luthman H, Olsson T. Genome-wide linkage analysis of chronic relapsing experimental autoimmune encephalomyelitis in the rat identifies a major susceptibility locus on chromosome 9. J Immunol. 1999 Mar 1;162(5):2581-8. PMID: 10072499. Jagodic M, Colacios C, Nohra R, Dejean AS, Beyeen AD, Khademi M, Casemayou A, Lamouroux L, Duthoit C, Papapietro O, Sjöholm L, Bernard I, Lagrange D, Dahlman I, Lundmark F, Oturai AB, Soendergaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV, Sadovnick DA, Ebers GC, Seddighzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Fournié GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple sclerosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID: 20368159. Colacios C, Casemayou A, Dejean AS, Gaits-Iacovoni F, Pedros C, Bernard I, Lagrange D, Deckert M, Lamouroux L, Jagodic M, Olsson T, Liblau RS, Fournié GJ, Saoudi A. The p.Arg63Trp polymorphism controls Vav1 functions and Foxp3 regulatory T cell development. J Exp Med. 2011 Oct 24;208(11):2183-91. Epub 2011 Sep 26. PMID: 21948080.
Additional strain information