Strain information  NBRP Rat No: 0676  Strain name: TRM.W-Tg(RNB1-186G14)33Kyo  Commmon Name: TRM Spata22 Tg Line33 Rat Genome Database Principal Investigator:  Takashi Kuramoto  Graduate School of Medicine, Kyoto University       Yoshidakonoe-cho, Sakyo-ku    606-8501 Kyoto     Japan Tel: 075-753-4494    Fax: 075-753-4409 Email: kuramot@anim.med.kyoto-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino Inbred Generations  N3F5 Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Origin F344/Stm rat BAC clone RNB1-186G14 was microinjected into embyos of Wistar rats. The founder rats were backcrossed to TRM rats. BAC vector:pKS145. RNB1-186G14 contains region between 60185236 - 60354841 of Rat Chr 10. This region contains Spata22 gene expressed in testes. Strain characteristics TRM rat has a 200-bp deletion including Aspa and Spata22 gene, resulting in leukodystrophy (Aspa defect) and infertility (Spata22 defect). By introduction of RNB1-186G14, infertility phenotype was rescued. Breeding Conditions crossing between tm/tm homozygous rats Genotyping Primers pKS145-F:aacgtcgtgactgggaaaac, pKS145-R:cccagcttctgtatggaacg PCR (2ｘAmpdirect,BIO TAQ DNA polymerase): 94℃ 3 min, 40 cycles of 94℃ 30 sec, 55℃ 1 min, 72℃ 45 sec, 72℃ 3 min, 15℃ 30 min References Additional strain information