Strain information

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NBRP Rat No: 0676

Strain name: TRM.W-Tg(RNB1-186G14)33Kyo

Commmon Name: TRM Spata22 Tg Line33

Rat Genome Database

Principal Investigator:

Takashi KuramotoTokyo University of Agriculture

Preservation Status:

Embryo Sperm Living Animals

Coat Color

albino

Inbred Generations

N3F5

Usage Restrictions

Genetic Status

Inbred Segregating Congenic Consomic Recombinant Coisogenic Spont. Mutant Transgene Ind. Mutant Category Other

Comercial Availability

Research Category

Diabetes Obesity Neurobiology Ophthalmology Dentistry Cardio Hypertension Cancer Metabolism Otorhinology Immunology Infectious Osteosis Internal Organ Dermatology Reproduction Development Behavior Hematology Urology Pharmacology Research Area Others

Control Strain Marker Strain

Gene Affected

Origin

F344/Stm rat BAC clone RNB1-186G14 was microinjected into embyos of Wistar rats. The founder rats were backcrossed to TRM rats. BAC vector:pKS145. RNB1-186G14 contains region between 60185236 - 60354841 of Rat Chr 10. This region contains Spata22 gene expressed in testes.

Strain characteristics

TRM rat has a 200-bp deletion including Aspa and Spata22 gene, resulting in leukodystrophy (Aspa defect) and infertility (Spata22 defect). By introduction of RNB1-186G14, infertility phenotype was rescued.

Breeding Conditions

crossing between tm/tm homozygous rats

Genotyping

Primers pKS145-F:aacgtcgtgactgggaaaac, pKS145-R:cccagcttctgtatggaacg PCR (2ｘAmpdirect,BIO TAQ DNA polymerase): 94℃ 3 min, 40 cycles of 94℃ 30 sec, 55℃ 1 min, 72℃ 45 sec, 72℃ 3 min, 15℃ 30 min

References

Additional strain information (Japanese only)