Strain information
 NBRP Rat No: 0695  Strain name: W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina  Commmon Name: Dox-dependent re-programmable rat, W-Tg(TRE-mOKS Ubc-rTet I/EGFP)T1-3/Hina Rat Genome Database
Principal Investigator:  Hiromitsu Nakauchi  The University of Tokyo, The Institute of Medical Science       4-6-1 Shirokanedai, Minato-ku,    108-8639 Tokyo     Japan
Tel: 03-5449-5331    Fax: 03-5449-5451 Email: nakauchi@ims.u-tokyo.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  
Usage Restrictions   The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.

In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Hamanaka S, st al., Generation of germline-competent rat induced pluripotent stem cells.　 PLoS One. 2011;6(7):e22008. Epub 2011 Jul 15

In publishing of data resulting from the use of the BIOLOGICAL RESOURCE, DEPOSITOR requires the statement that the BIOLOGICAL RESOURCE belongs to JST ERATO, NAKAUCHI Stem Cell and Organ Regeneration.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 初期化誘導
 Control Strain  Marker Strain
Gene Affected Doxycycline誘導発現型マウスOct3/4, Klf4, Sox2、Ubc promoter下流EGFP
Origin Doxycycline induced mouse Oct3/4, Klf4, Sox2/ Ubc-promoter EGFP was introduced into embryonic fibroblast of Wistar rat (Slc:Wistar）by replication incompetent type lentivirus vector and iPS cells were induced. Chimeric rats (male) were generated from this iPS cells, and crossed with the wild-type Wistar rat (female).
Strain characteristics The reprogramming can be induced by re-induction of transgene (Oct3/4, Klf4, Sox2) in the presence of Doxycycline. iPS cells can be induced from somatic cells under the optimal culture condition.
Breeding Conditions Maintained in heterozygous condition, Good breeding performance. About a half of rats has EGFP expression.
Genotyping
References ・Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H.
Generation of germline-competent rat induced pluripotent stem cells.　 PLoS One. 2011;6(7):e22008. Epub 2011 Jul 15

・Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.PLoS One. 2012;7(7):e41007. doi: 10.1371/journal.pone.0041007. Epub 2012 Jul 18.
Additional strain information