Strain information
NBRP Rat No: 0678
Strain name
: TRMR.W-Tg(RNB1-186G14)33Kyo
Commmon Name: TRMR Spata22 Tg Line33
Rat Genome Database
Principal Investigator:
Takashi Kuramoto Graduate School of Medicine, Kyoto University Yoshidakonoe-cho, Sakyo-ku 606-8501 Kyoto Japan
Tel: 075-753-4494 Fax: 075-753-4409
Email: kuramot@anim.med.kyoto-u.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
albino
Inbred Generations
N4F6
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Origin
F344/Stm rat BAC clone RNB1-186G14 was microinjected into embyos of Wistar rats. The founder rats were backcrossed to TRMR rats. BAC vector:pKS145. RNB1-186G14 contains region between 60185236-60354841 of Rat Chr 10 and this region contains Spata22 gene expressed in testes.
Strain characteristics
TRM rat has a 200-bp deletion including Aspa and Spata22 gene, resulting in leukodystrophy (Aspa defect) and infertility (Spata22 defect). By introduction of RNB1-186G14, infertility phenotype was rescued.
Breeding Conditions
Genotyping
primers pKS145-F:aacgtcgtgactgggaaaac、pKS145-R:cccagcttctgtatggaac
References
Additional strain information