| NBRP Rat No: 0698 |
Strain name: DA.PVG.1AV1-(OT24.18-D10Rat93)/Kini |
Commmon Name: DA.PVG.1AV1-Eae18b |
Rat Genome Database |
| Principal Investigator: |
Tomas Olsson Karolinska Institutet CMM, Karolinska University Hospital, 171 76, L8:04, Stockholm Sweden |
| Tel: +46 8 51776258 Fax: +46 8 51775562 |
Email: tomas.olsson@ki.se |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
Agouti |
| Inbred Generations |
Congenic strain was establiesh from the 10th generation advanced intercross line after 8 generations of backcrossing in 2008. Since then, the strain was kept as inbred. |
| Usage Restrictions |
In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in this article:
Adzemovic MZ, Ockinger J, Zeitelhofer M, Hochmeister S, Beyeen AD, Paulson A, Gillett A, Hedreul MT, Covacu R, Lassmann H, Olsson T, Jagodic M. Expression of Ccl11 Associates with Immune Response Modulation and Protection against Neuroinflammation in Rats. PLoS One. 2012;7(7):e39794. Epub 2012 Jul 16. PMID: 22815714. |
| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
> 13.5 Mb |
| Origin |
Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Animals for congenic breeding were selected from the 10th generation of an advanced intercross line (AIL) originating from the EAE-susceptible DA and EAE-resistant PVG.av1 rat strains. Selected males containing a PVG.av1 fragment in the Eae18b region (from OT24.18 to D10Rat93 marker, at 68.36 Mb and 81.9 Mb, respectively) were backcrossed with DA females for 8 generations, and offspring was genotyped using microsatellite markers in each generation. In the 8th generation, heterozygous males and females were crossed to obtain the experimental population of homozygous congenic rats and littermate controls. |
| Strain characteristics |
Protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain and displays higher CCL11 expression. |
| Breeding Conditions |
Good breeding performance. |
| Genotyping |
Flanking markers inside the congenic fragment: OT24.18-D10Rat93. |
| References |
Jagodic M, Becanovic K, Sheng JR, Wu X, Bäckdahl L, Lorentzen JC, Wallström E, Olsson T. An advanced intercross line resolves Eae18 into two narrow quantitative trait loci syntenic to multiple sclerosis candidate loci. J Immunol. 2004 Jul 15;173(2):1366-73. PMID: 15240732.
Ockinger J, Stridh P, Beyeen AD, Lundmark F, Seddighzadeh M, Oturai A, Sørensen PS, Lorentzen AR, Celius EG, Leppä V, Koivisto K, Tienari PJ, Alfredsson L, Padyukov L, Hillert J, Kockum I, Jagodic M, Olsson T. Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis. Genes Immun. 2010 Mar;11(2):142-54. Epub 2009 Oct 29. PMID: 19865101.
Adzemovic MZ, Ockinger J, Zeitelhofer M, Hochmeister S, Beyeen AD, Paulson A, Gillett A, Hedreul MT, Covacu R, Lassmann H, Olsson T, Jagodic M. Expression of Ccl11 Associates with Immune Response Modulation and Protection against Neuroinflammation in Rats. PLoS One. 2012;7(7):e39794. Epub 2012 Jul 16. PMID: 22815714. |
| Additional strain information |
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