Strain information
NBRP Rat No: 0717
Strain name
: LEA-Tg(Pou5f1-YFP*)3Ncco
Commmon Name: Oct4-Venus Tg rat, LEA-esTgN(Oct4/Venus)3NCCRI
Rat Genome Database
Principal Investigator:
Takahiro Ochiya Tokyo Medical University Institute of Medical Science 6-7-1 Nishi-Shinjuku, Shinjyuku-ku, 160-0023 Tokyo Japan
Tel: 03-3342-6111 Fax:
Email: tochiya@tokyo-med.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
茶色
Inbred Generations
不明
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The recipient is requested to cite the literature designated by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Oct4 (Pou5f1: POU class 5 homeobox 1 ) promoter, Venus (YFP*: Improved version of yellow fluorescent protein)
Origin
This strain was provided from Kyoto Bioresource center.
Strain characteristics
Venus fluorescence can be detected in Oct4(Pou5f1)-expressing cells (especially in germ cell and ES cells)
Breeding Conditions
Mating between Tg rat and wild-type LEA rat.
Genotyping
PCR analysis, 200-bp Forward: ACCCTGAAGCTGATCTGCAC Reverse: GGTCTTGTAGTTGCCGTCGT KOD version2 94℃: 2 min 98℃: 20 sec 62℃: 20 sec 68℃: 30 sec (35 cysles) 68℃: 5 min 4℃
References
Kawamata M, Ochiya T. Generation of genetically modified rats from embryonic stem cells. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14223-8.
Kawamata M, Ochiya T. Two distinct knockout approaches highlight a critical role for p53 in rat development. Sci Rep 2012 2: 945
Additional strain information