Strain information
 NBRP Rat No: 0734  Strain name: LE-Tg(Gt(ROSA)26Sor-CAG-tdTomato)24Jfhy  Commmon Name: Tg(RP23-244D9/CAG-neo-tdTomato)Jfhy, LE-Tg(Gt(ROSA)26Sor/CAG-tdTomato)24Jfhy Rat Genome Database
Principal Investigator:  Hiromu Yawo  Tohoku University       2-1-1, Katahira, Aoba-ku, Sendai-shi    980-8577 Miyagi     Japan
Tel: 022-217-6208    Fax: 022-217-6211 Email: hiromu.yawo.c7@tohoku.ac.jp （担当　五十嵐 敬幸：hirobio@med.tohoku.ac.jp）
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白黒
Inbred Generations  F2
Usage Restrictions  Any form of publications of data resulting from the use of the BIOROGICAL RESOURCE should reference the following DEPOSITOR’s papers.
“Igarashi, H., Koizumi, K., Kaneko, R., Ikeda, K., Egawa, R., Yanagawa, Y., Muramatsu, S., Onimaru, H., Ishizuka, T., Yawo, H. A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato. PLoS One. 11(5), e0155687 (2016)”
In publishing, an acknowledgment to the DEPOSITOR is requested.
The BIOROGICAL RESOURCE should be used under the following rules.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOUCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others レポーターラットとして、発生学、脳神経科学、Creドライバーラットキャラクタリゼーションに有用である
 Control Strain  Marker Strain
Gene Affected CAG-loxP-flanked Neo/STOP cassette-tdTomato
Origin Background strain: LongEvans Transgene: CAG-loxP-flanked Neo/STOP cassette-tdTomato was inserted into mouse ROSA26 BAC (RP23 244D9). The inserted gene copy nymber is one copy.
Strain characteristics tdTomato (red fluorescence) expression is detected in the presence of Cre recombinase.
Breeding Conditions crossing with the wild-type Long-Evans rat
Genotyping Detection of neo sequence between loxP secuences by PCR analysis. Primers: Forward ctatgactgggcacaacagacaat, Reverse aactcgtcaagaaggcgatagaag PCR condition (KOD Fx neo): 94℃　2min, 30 cycles of 98℃ 10s and 68℃　60s, 4℃ Product size: 726bp
References Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S, Onimaru H, Ishizuka T, Yawo H.
A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein TdTomato.
PLoS One. 2016 May 19;11(5):e0155687.

Soma S, Yoshida J, Kato S, Takahashi Y, Nonomura S, Sugimura YK, Ríos A,
Kawabata M, Kobayashi K, Kato F, Sakai Y, Isomura Y. Ipsilateral-Dominant Control
of Limb Movements in Rodent Posterior Parietal Cortex. J Neurosci. 2019 Jan
16;39(3):485-502.
Additional strain information