Strain information
 NBRP Rat No: 0756  Strain name: F344.GRY-Cacna1agryScn1am1Kyo/Okym  Commmon Name: RGD: F344-Cacna1agryScn1am1Kyo/Okym Rat Genome Database
Principal Investigator:  Iori Ohmori  Okayama University       Tsushima 3-chome 1-1, Kita-ku, Okayama    700-8530      Japan
Tel:     Fax:  Email: iori@md.okayama-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  F23
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Cacna1a-M251K ヘテロ　Scn1a-N1417H　ホモ
Origin GRY/Idr (GRY/Idr has the M251K mutation in the Cacna1a gene) was backcrossed to F344/NSlc, and then crossed to HISS rat (HISS rat has the N1417H mutation in the Scn1a gene) to generate Scn1a/Cacna1a double mutant rats.
Strain characteristics Homo double mutant rats are smaller in size than each single mutant rat. This strain has cerebellar ataxia and walks with its hind limbs wobbling in a wide position. This strain is complicated by epileptic seizures, with absence and myoclonic seizures and seizures similar to partial seizures. In addition, the cramp threshold for hyperthermia is low.
Breeding Conditions Homo double mutant rats, both males and females, are unable to reproduce by natural mating. Cacna1a hetero mutant rats and Scn1a homo mutant rats must be crossed to create homo double mutant rats.
Genotyping Primers: Forward 5'-TCTCTGTCTCCCCAGGTTTAC-3', Reverse 5'-GTGGCTAACACACAGCTTTGC-3' PCR analysis: 94℃ 3 min, 40 cycles of 94℃ 30 sec/ 64℃ 1 min/ 72℃ 1 min, and 72℃ 7 min Digestion of PCR products by PsiI (37℃, 2 hrs).
References Scn1a and Cacna1a mutations mutually alter their original phenotypes in rats.
Ohmori I, Kobayashi K, Ouchida M.
Neurochemistry International, 2020.
Additional strain information