NBRP Rat No: 0739
Commmon Name: F344-Depdc5em2Kyo/Kyo
Rat Genome Database
Stephanie BAULAC ICM - Institut du Cerveau et de la Moelle épinière Hôpital Pitié-Salpêtrière 47 bd de l'Hôpital 75013 Paris France
Tel: +33 (0)1-5727-4339 Fax: +33 (0)1-5727-4339
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Research Area Others
Depdc5: DEP domain containing 5
This rat was created by the transcription activator-like effector nuclease (TALEN) technology. A pair of TALENs targeting exon 2 of rat Depdc5 (Ensembl: ENSRNOT00000085788) were microinjected into fertilized eggs of F344, and then transferred into the oviducts of pseudopregnant Wistar female rats, as previously described (Mashimo et al., 2013). TAL Effector Nucleotide Targeter 2.0 (https://tale-nt.cac.cornell.edu/) did not predict off-target site.
Homozygous Depdc5−/− embryos died from embryonic day 14.5 due to a global growth delay. Heterozygous Depdc5+/− rats developed normally and exhibited no spontaneous electroclinical seizures, but had altered cortical neuron excitability and firing patterns. Depdc5+/− rats displayed cortical cytomegalic dysmorphic neurons and balloon-like cells strongly expressing phosphorylated rpS6, indicative of mTORC1 upregulation, and not observed after prenatal rapamycin treatment.
Homozygous Depdc5−/− rats are lethal, but heterozygous Depdc5+/− rats are fertile.
Primer (5'-3') Forward AGCCTGACATTCTCGCTGTT Reverse TCTTGCCCCACTCATTTACC (product size 267bp) Mutation c.39_55delinsT (p.Lys13Asnfs*8) (NM_001107229.1)
Marsan E, Ishida S, Schramm A, Weckhuysen S, Muraca G, Lecas S, Liang N, Treins C, Pende M, Roussel D, Le Van Quyen M, Mashimo T, Kaneko T, Yamamoto T, Sakuma T, Mahon S, Miles R, Leguern E, Charpier S, Baulac S.
Depdc5 knockout rat: A novel model of mTORopathy.
Neurobiol Dis. 2016 Feb 9. pii: S0969-9961(16)30031-6.
Additional strain information