Strain information
 NBRP Rat No: 0755  Strain name: F344.GRY-Cacna1agry/Okym  Commmon Name: M251K-Cacna1a mutation, RGD: F344-Cacna1agry/Okym Rat Genome Database
Principal Investigator:  Iori Ohmori  Okayama University       Tsushima 3-chome 1-1, Kita-ku, Okayama    700-8530      Japan
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Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白
Inbred Generations  F23
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Cacna1a
Origin This strain is derived from GRY/Idr (GRY/Idr has the M251K mutation in the Cacna1a gene) provided from NBRP-Rat. GRY/Idr was backcrossed to F344/NSlc.
Strain characteristics The homozygous rats show absence epilepsy and cerebellar ataxia. No significant physical abnormality is observed in the heterozygous rats.
Breeding Conditions Mating between heterozygous male and heterozygous female Homozygous female rats tend to neglect their pups.
Genotyping Primers: Forward 5’-TCTCTGTCTCCCCAGGTTTAC-3', Reverse 5’-GTGGCTAACACACAGCTTTGC-3’ PCR analysis: 94℃ 3 min, 40 cycles of 94℃ 30 sec/ 64℃ 1 min/ 72℃ 1 min, and 72℃ 7 min Digestion of PCR products by PsiI (37℃, 2 hrs).
References Scn1a and Cacna1a mutations mutually alter their original phenotypes in rats.
Ohmori I, Kobayashi K, Ouchida M.
Neurochemistry International, 2020.
Additional strain information