NBRP Rat No: 0755 |
Strain name: F344.GRY-Cacna1agry/Okym |
Commmon Name: M251K-Cacna1a mutation, RGD: F344-Cacna1agry/Okym |
Rat Genome Database |
Principal Investigator: |
Iori Ohmori Okayama University Tsushima 3-chome 1-1, Kita-ku, Okayama 700-8530 Japan |
Tel: Fax: |
Email: iori@md.okayama-u.ac.jp |
Preservation Status: |
Embryo Sperm Living Animals |
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Coat Color |
白 |
Inbred Generations |
F23 |
Usage Restrictions |
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Genetic Status |
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Comercial Availability |
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Research Category |
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Gene Affected |
Cacna1a |
Origin |
This strain is derived from GRY/Idr (GRY/Idr has the M251K mutation in the Cacna1a gene) provided from NBRP-Rat.
GRY/Idr was backcrossed to F344/NSlc. |
Strain characteristics |
The homozygous rats show absence epilepsy and cerebellar ataxia. No significant physical abnormality is observed in the heterozygous rats. |
Breeding Conditions |
Mating between heterozygous male and heterozygous female
Homozygous female rats tend to neglect their pups. |
Genotyping |
Primers: Forward 5’-TCTCTGTCTCCCCAGGTTTAC-3', Reverse 5’-GTGGCTAACACACAGCTTTGC-3’
PCR analysis: 94℃ 3 min, 40 cycles of 94℃ 30 sec/ 64℃ 1 min/ 72℃ 1 min, and 72℃ 7 min
Digestion of PCR products by PsiI (37℃, 2 hrs). |
References |
Scn1a and Cacna1a mutations mutually alter their original phenotypes in rats.
Ohmori I, Kobayashi K, Ouchida M.
Neurochemistry International, 2020. |
Additional strain information |
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