Strain information

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NBRP Rat No: 0755

Strain name: F344.GRY-Cacna1a^gry/Okym

Commmon Name: M251K-Cacna1a mutation, RGD: F344-Cacna1a^gry/Okym

Rat Genome Database

Principal Investigator:

Iori OhmoriOkayama University

Preservation Status:

Embryo Sperm Living Animals

Coat Color

白

Inbred Generations

F23

Usage Restrictions

In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Genetic Status

Inbred Segregating Congenic Consomic Recombinant Coisogenic Spont. Mutant Transgene Ind. Mutant Category Other

Comercial Availability

Research Category

Diabetes Obesity Neurobiology Ophthalmology Dentistry Cardio Hypertension Cancer Metabolism Otorhinology Immunology Infectious Osteosis Internal Organ Dermatology Reproduction Development Behavior Hematology Urology Pharmacology Research Area Others

Control Strain Marker Strain

Gene Affected

Cacna1a

Origin

This strain is derived from GRY/Idr (GRY/Idr has the M251K mutation in the Cacna1a gene) provided from NBRP-Rat. GRY/Idr was backcrossed to F344/NSlc.

Strain characteristics

The homozygous rats show absence epilepsy and cerebellar ataxia. No significant physical abnormality is observed in the heterozygous rats.

Breeding Conditions

Mating between heterozygous male and heterozygous female Homozygous female rats tend to neglect their pups.

Genotyping

Primers: Forward 5’-TCTCTGTCTCCCCAGGTTTAC-3', Reverse 5’-GTGGCTAACACACAGCTTTGC-3’ PCR analysis: 94℃ 3 min, 40 cycles of 94℃ 30 sec/ 64℃ 1 min/ 72℃ 1 min, and 72℃ 7 min Digestion of PCR products by PsiI (37℃, 2 hrs).

References

Scn1a and Cacna1a mutations mutually alter their original phenotypes in rats.
Ohmori I, Kobayashi K, Ouchida M.
Neurochemistry International, 2020.

Additional strain information (Japanese only)