Strain information
 NBRP Rat No: 0771  Strain name: WKY-Tg(UBC-sb10)3Wmukf  Commmon Name: USB, WKYO-Tg(UBC-SleepingBeauty) Rat Genome Database
Principal Investigator:  Richard R. Behringer  The University of Texas MD Anderson Cancer Center       1515 Holcombe Blvd,Houston, TX 77030    77030 Houston     USA
Tel: 713-834-6327    Fax:  Email: rrb@mdanderson.org
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  albino
Inbred Generations  F9
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. "Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat. Furushima K, Jang CW, Chen DW, Xiao N, Overbeek PA, Behringer RR.
Genetics. 2012 Dec;192(4):1235-48."
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The material (rat) is also authorized by Kenryo Furushima. Regardless of the profit and non-profit purpose, the applicant is allowed to use the material (rat) when the applicant shows the purpose to use the material and Kenryo Furushima agrees with the purpose and the applicant obtains a permission letter from Kenryo Furushima. Any products and results obtained from the research of non-commercial purpose belong to the applicant. When applicant obtains a patent using any products and results from the rats or uses them for profit-making purpose, the applicant is allowed to perform them after Kenryo Furushima and Wakayama Medical University agree with the purpose. Contact (E-mail) clear.out.add@gmail.com
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others random mutagenesis
 Control Strain  Marker Strain
Gene Affected UBC (ubiquitin C), SleepingBeauty transposase
Origin This strtain has tasnsgene that express SleepingBeauty transposase under human Ubiquitin-C promoter. (insertion site of trans gene: unknown)
Strain characteristics Systemic expression of Sleeping Beauty transposase. This Tg rats grow normally and have normal breeding performance. Background strain: Wistar Kyoto（NTac:WKY, Taconic Biosciences, Inc. )
Breeding Conditions mating between transgenic rats and Wistar/Kyo strain rats
Genotyping ＜PCR method＞ (UbC-F) 5'-CTGAAGCTCCGGTTTTGAACTATGCG-3' (SB-R) 5'-GTACAGATGAACGTGGTACCGCCAG-3' amplified size: 351bp
References Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat.
Furushima K, Jang CW, Chen DW, Xiao N, Overbeek PA, Behringer RR.
Genetics. 2012 Dec;192(4):1235-48.
Additional strain information