NBRP Rat No: 0778 |
Strain name: W-Tg(HCRT-cre,-EGFP)B5Ahky |
Commmon Name: orexin-Cre rat |
Rat Genome Database |
Principal Investigator: |
Akihiro Yamanaka Nagoya University Furo-cho, Chikusa-ku 464-8601 Nagoya Japan |
Tel: 052-789-3864 Fax: 052-789-3889 |
Email: yamank@riem.nagoya-u.ac.jp |
Preservation Status: |
Embryo Sperm Living Animals |
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Coat Color |
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Inbred Generations |
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Usage Restrictions |
The RECIPIENT should inform DEPOSITOR about research project in advance. DEPOSITOR should be included as co-authors (at least in the first article). |
Genetic Status |
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Comercial Availability |
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Research Category |
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Gene Affected |
HCRT: hypocretin (orexin) neuropeptide precursor , EGFP: Enhanced Green Fluorescent Protein |
Origin |
This transgenic rat strain was established by Dr. Yamanaka (Nagoya University) in 2012. Background strain: Wistar rat (CLEA Japan). plasmid backbone: pCR2.1. Transgene: human HCRT gene promoter, cre recombinase, EGFP gene, 2A gene |
Strain characteristics |
EGFP and cre recombinase are specifically expressed in orexin neurons. |
Breeding Conditions |
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Genotyping |
Genotyping by PCR. Primers for EGFP: EGFP5 CACAAGTTCAGCGTGTCCG, EGFP32 TCAGCTCGATGCGGTTCAC or primers for hOX-promoter: hoxpro-AS AAGTCGACGGTGTCTGGCGCTCAGGGTG, hoxpro-S GCAGCGGCCATTCCTTGG |
References |
Dergacheva O, Yamanaka A, Schwartz AR, Polotsky VY, Mendelowitz D.
Hypoxia and hypercapnia inhibit hypothalamic orexin neurons in rats.
J Neurophysiol. 2016 Nov 1;116(5):2250-2259. doi: 10.1152/jn.00196.2016. Epub 2016
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Additional strain information |
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