NBRP Rat No: 0763
Commmon Name: SKOrat
Rat Genome Database
Kazuwa Nakao Kyoto University Graduate School of Medicine 53 Shogoin-Kawahara-chou, Sakyo-ku 606-8507 Kyoto city Japan
Tel: 075-366-7450 Fax: 075-752-7140
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from DEPOSITOR. The RECIPIENT must agree on collaborative research with the DIPOSITOR. Any form of publication data resulting from the use of the BIOLOGICAL RESOURCE require co-authorship. This condition is NOT limited to two-year period after deposition.
Research Area Others
T239A mutation was found by screening of 4608DNA sample from ENU mutagenesis library KURMA. This strain has a T239A (L80X) nonsense mutation in Bscl2 gene.
Bscl2T239A homozygous mutated rat shows severe atrophy of adipose tissue, hypoleptinemia, abnormal glucose tolerance, increment of triglyceride levels in the liver. Bscl2T239A mutated male rats have small testes with sperm hypoplasia and are infertile.
crossing between heterozygous rats
Primers: Forward 5’-TGTGGGCCCAGGAAGTG-3’, Reverse 5’-CCCCAAACTGCAGCATCAG-3’ TaqMan MGB probes: WT probe 5’-CCTGCCAACACATGG-3’, mutant probe 5’-CCTGCCTACACATGG-3’ PCR condition: 95C 20 sec, 20 cycles od 95C 3 sec and 60C 20 sec
Ebihara C, Ebihara K, Aizawa-Abe M, Mashimo T, Tomita T, Zhao M, Gumbilai V, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K.
Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.
Hum Mol Genet. 2015 May 1. pii: ddv156.
Additional strain information