Strain information
 NBRP Rat No: 0763  Strain name: F344-Bscl2m1Kyo  Commmon Name: SKOrat Rat Genome Database
Principal Investigator:  Kazuwa　Nakao  Kyoto University Graduate School of Medicine        53 Shogoin-Kawahara-chou, Sakyo-ku    606-8507 Kyoto city     Japan
Tel: 075-366-7450    Fax: 075-752-7140 Email: nakao@kuhp.kyoto-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白
Inbred Generations  N10 (2014.09.10)
Usage Restrictions  The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from DEPOSITOR. The RECIPIENT must agree on collaborative research with the DIPOSITOR. Any form of publication data resulting from the use of the BIOLOGICAL RESOURCE require co-authorship. This condition is NOT limited to two-year period after deposition.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Bscl2
Origin T239A mutation was found by screening of 4608DNA sample from ENU mutagenesis library KURMA. This strain has a T239A (L80X) nonsense mutation in Bscl2 gene.
Strain characteristics Bscl2T239A homozygous mutated rat shows severe atrophy of adipose tissue, hypoleptinemia, abnormal glucose tolerance, increment of triglyceride levels in the liver. Bscl2T239A mutated male rats have small testes with sperm hypoplasia and are infertile.
Breeding Conditions crossing between heterozygous rats
Genotyping Primers: Forward 5’-TGTGGGCCCAGGAAGTG-3’, Reverse 5’-CCCCAAACTGCAGCATCAG-3’ TaqMan MGB probes: WT probe 5’-CCTGCCAACACATGG-3’, mutant probe 5’-CCTGCCTACACATGG-3’ PCR condition:　95C 20 sec, 20 cycles od 95C 3 sec and 60C 20 sec
References Ebihara C, Ebihara K, Aizawa-Abe M, Mashimo T, Tomita T, Zhao M, Gumbilai V, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K.
Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.
Hum Mol Genet. 2015 May 1. pii: ddv156.
Additional strain information