Strain information
NBRP Rat No: 0769
Strain name
: F344-
Asip
A
Tyr
C
Kit
H
/Kyo
Commmon Name: Agouti Fischer
Rat Genome Database
Principal Investigator:
Tomoji Mashimo The University of Tokyo, The Institute of Medical Science 4-6-1 Shirokanedai-Minatoku-Tokyoto 108-8639 Japan
Tel: 03-6409-2489 Fax:
Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
Inbred Generations
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Origin
1) point mutation in the exon 2 of Tyrosinase (Tyr) gene (albino phenotype), 2) 19-bp deletion in Asip gene (Agouti phenotype), and 3) retrotransposon insertion (7-bp) in kit gene (hooded phenotype) were targeted by CRISPR/Cas9 system using ssODN. The rat coat colour phenotype was changed from white to Agouti. Background strain: F344/Stm
Strain characteristics
Agouti coat colour (genetic background: F344/Stm)
Breeding Conditions
homozygous condition (wild-type color: Agouti)
Genotyping
Tyr gene can be distinguished by sequence analysis (primers：TTGCATAAATTGGTTTTCACAGA and ATTTAAACATGAAAATATTACCTTCCA) Asip gene can be distinguished by PCR and AGE analysis (primers：ATGTCACCCGCTTACTCCTG and ACACTGCCAAGGACGCTATT) Kit gene can be distinguished by PCR and AGE analysis (primers：ACTCCAGGCCACAGACAACT and TGAAGAAAATTAGCATACCCGTCT)
References
Yoshimi K, Kaneko T, Voigt B, Mashimo T.
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform.
Nat Commun. 2014 Jun 26;5:4240.
Additional strain information