Strain information
NBRP Rat No: 0770
Strain name
: W-
Rosa26
em1(CAG-EGFP)Kyo
Commmon Name: Rosa EGFP knockin
Rat Genome Database
Principal Investigator:
Tomoji Mashimo The University of Tokyo, The Institute of Medical Science 4-6-1 Shirokanedai-Minatoku-Tokyoto 108-8639 Japan
Tel: 03-6409-2489 Fax:
Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
Inbred Generations
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Origin
CAG－GFP vector was knock-in into the rat Rosa26 locus by CRISPR/Cas9 system. Background strain: Crlj:WI
Strain characteristics
Strong and stable systemic EGFP expression under CAG-promoter in the rat Rosa26 locus
Breeding Conditions
maintained in EGFP-homozygous condition
Genotyping
primers： rRosa26_Small_F : AAGGGAGCTGCAGTGGAGTA and rRosa26_Small_R : CCCAGGTGAGTGCCTAGTCT (360-bp) GFP expression is checked by phenotype. GFP_Small_F : CTACCCCGACCACATGAAG and GFP_Small_R : CTTGTGCCCCAGGATGTT "
References
Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T.
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Nat Commun. 2016 Jan 20;7:10431
Additional strain information