Strain information
 NBRP Rat No: 0779  Strain name: WIC-Dmdem1Kykn  Commmon Name: Dystrophin mutated rat (Out of Frame-1) Rat Genome Database
Principal Investigator:  Keitaro Yamanouchi  The University of Tokyo        1-1-1, Yayoi, Bunkyo-ku,    113-8657 Tokyo     Japan
Tel: 03-5841-5386    Fax: 03-5841-8017 Email: akeita@g.ecc.u-tokyo.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白（アルビノ）
Inbred Generations  F1
Usage Restrictions  In publishing, a citation of the following literatures designated by the DEPOSITOR is requested.
Nakamura K, Fujii W, Tsuboi M, Tanihara J, Teramoto N, Takeuchi S, Naito K, Yamanouchi K, Nishihara M, Generation of muscular dystrophy model rats with a CRISPR/Cas system. Sci Rep 4: 5635(2014)
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 筋疾患
 Control Strain  Marker Strain
Gene Affected ジストロフィン (Dmd: dystrophin)
Origin By CRISPR/Cas9 system, mutation was introduced in dystrophin (Dmd) gene of Wistar-Imamichi rat (Iar: Wistar-Imamichi, Institute for Animal Reproduction). Two exons in the rat Dmd gene was targeted; 329-bp deletion around exon 3 Exon16: 5’-GCACAt*A(AT)#GG-3’ (*：single base insertion, #： 2-bp substitution)
Strain characteristics This rat exhibits similar phenotypes to Duchenne muscular dystrophy (DMD). Skeletal muscle degeneration from 4 weeks of age, myocardial necrosis and inflammation at 13 weeks, decrease in body weight from 6 month old
Breeding Conditions natural mating
Genotyping Set#1　（exon3） Primer 1F: AGTTTCCATCAATAGCCATACCAAA, Primer 1R: TCTCAGTGTACAAGTGTGACGAACA Set#2　（exon16) Primer 2F: TGGAAAACTTTGCACATAAT, Primer 2R: AAGCTTGGAGAGGAAGTTGAAGACT PCR condition: 94℃ 5 min, 98℃ 10 sec 58℃ 30 sec 68℃ 40 sec (38 Cycles) DMD rat: Set＃1 about 500 bp, Set#２：about 500 bp WT rat: Set＃1 about 1 kb, Set#２ no band heterozygous female:Set＃1 two bands (about 1 kb and 500 bp), Set#２ about 500 bp
References Nakamura K, Fujii W, Tsuboi M, Tanihata J, Teramoto N, Takeuchi S, Naito K, Yamanouchi K, Nishihara M.
Generation of muscular dystrophy model rats with a CRISPR/Cas system.
Sci Rep 4: 5635 (2014)
Additional strain information