Strain information
 NBRP Rat No: 0781  Strain name: WIC-Dmdem3Kykn  Commmon Name: Dystrophin mutated rat (In-Frame-2) Rat Genome Database
Principal Investigator:  Keitaro Yamanouchi  The University of Tokyo        1-1-1, Yayoi, Bunkyo-ku,    113-8657 Tokyo     Japan
Tel: 03-5841-5387    Fax: 03-5841-8017 Email: akeita@mail.ecc.u-tokyo.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白（アルビノ）
Inbred Generations  F1
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 筋疾患
 Control Strain  Marker Strain
Gene Affected ジストロフィン (Dmd: dystrophin)
Origin By CRISPR/Cas9 system, mutation was introduced in dystrophin (Dmd) gene of Wistar-Imamichi rat: Deletion of exon3 to exon16 (a part of intron 3 remain). This IF2 strain （W-Dmdem3Kykn）died out in 2015.
Strain characteristics This rat exhibits similar phenotypes to Becker muscular dystrophy(BMD): focal skeletal muscle necrosis and regeneration at 8 weeks of age and muscular hypertrophy at 8 months of age in affected male rats.
Breeding Conditions In male rats, spermatogenesis is not observed (infertility). This IF2 strain （W-Dmdem3Kykn）died out in 2015.
Genotyping Primer F: AAAAGGAGAACAGGAGTTTTTGAAT Primer R: TACAGTTTTCCATTTCTGAAGAACC 94℃ 5 min 98℃ 10 sec 58℃ 30 sec 68℃ 40 sec (38 Cycles) KOD FX Neo (/sample) 2x Buffer 10 μl water 4 μl DNTPs 4 μl Primer F 0.6 μl R 0.6 μl Template 1 μl KOD 0.4 μl IF-2：2 kb band wild：no band heterozygous female：2 kb band
References Nakamura K, Fujii W, Tsuboi M, Tanihata J, Teramoto N, Takeuchi S, Naito K, Yamanouchi K, Nishihara M. Generation of muscular dystrophy model rats with a CRISPR/Cas system. Sci Rep 4: 5635 (2014)
Additional strain information