Strain information
NBRP Rat No: 0786
Strain name
: W-Tg(CMV-Ddx54)37Tsuy
Commmon Name: ヘリカーゼ遺伝子導入ラット（HELラット）, Wistar系ラット(37)
Rat Genome Database
Principal Investigator:
Yoshihiro Tsuruo Wakayama Medical University School of Medicine 811-1 Kimiidera 641-8509 Wakayama Japan
Tel: 073-441-0616 Fax: 073-441-0860
Email: tsuruo@wakayama-med.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白
Inbred Generations
31世代
Usage Restrictions
In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. [J Neurosci Res 90: 48-59 (2012), J Neurosci Res 91: 335-348 (2013)]
In publishing, an acknowledgment to the DEPOSITOR is requested.
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
A cooperative research is required (DEPOSITOR should be included as co-authors).
For academic research only.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
ラットヘリカーゼ Ddx54
Origin
This transgenic rat was established by microinjection of transgene consisting of Ddx54 gene (NM_001191548.1) driven by CMV promoter (pCMV-Tag2B) derived from cytomegalovirus into Wistar rat (SLC) pronuclear fertile eggs at UNITECH Co., Ltd. in 2007.
Strain characteristics
hypermyelination
Breeding Conditions
Mating in a standard manner. 2 lines (line 19 and 37) are maintained.
Genotyping
forward primerＡ 5’-GCGTTACATAACTTACGGTAAATGG-3 (pCMV-Tag2B), forward primerB 5’-ATGGCTGCCGGCAGACGTGTG-3' and reverse primerC 5’-ATCCTCTGCTTGGATCTCGAA-3' (Ddx54) Annealing temperature: 55℃ PCR product size is as follows; A-C: 800 bp, B-C: 150 bp Two bands (800 and 150 bp) are generated from the Tg rats, but only a single band (150 bp)is detected in the WT rats.
References
J Neurosci Res 90: 48-59 (2012) J Neurosci Res 91: 335-348 (2013)
Additional strain information