| NBRP Rat No: 0797 |
Strain name: WIC-Tg(Nanog-YFP*)1Utr |
Commmon Name: Iar:W-I-Tg(Nanog-Venus, -PuroP)1UTR, W-Tg(Nanog-YFP*)1Utr |
Rat Genome Database |
| Principal Investigator: |
Humihiro Sugiyama Tsukuba University 305-0006 Japan |
| Tel: 029-853-3384 Fax: 029-853-3380 |
Email: bunbun@md.tsukuba.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
Albino (c) |
| Inbred Generations |
F9 |
| Usage Restrictions |
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Investigattors are required to conclude MTA with the Laboratory Animal Resource Center, University of Tsukuba. |
| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
YFP* (Venus): yellow fluorescent protein variant, Nanog: Nanog homeobox |
| Origin |
BAC (RNB2-297P05, containing rat Nanog gene) vector was injected into Iar:Wistar-Imamichi rats. The construct is as follows: Venus-IRES-puromycin resistance gene. |
| Strain characteristics |
Venus (YFP*) fluorescence can be detected in the Nanog-expressing cells (inner cell mass of blastocyst
and primordial germ cells). |
| Breeding Conditions |
maintained by mating between Tg rat and wild type Iar:W-I |
| Genotyping |
Primers: 5'-CCGGAATTCATGACCGAGTACAAGCCCAC-3' および 5'-CCGGAATTCTCAGGCACCGGGCTTGCG-3'
PCR condition: GXL Taq (TAKARA) 98℃ 4min (98℃ 10sec, 60℃ 15sec, 68℃ 45sec)×33, 68℃ 3min. product size: 600 bp |
| References |
Mikhailov A, Sugiyama F, Mizuno S, and Sankai Y.
Bio-active polymer implants to adipose tissue as in situ source of reprogramming cells.
2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) 2016, Page(s):4169 - 4172 |
| Additional strain information |
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