Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Gene Affected
tTA: tetracycline transactivator, Pvalb: Parvalbumin
Origin
background strain：Long-Evans rat（Iar:Long-Evans）
Transgene：
(1) Parvalbumin (Pvalb) promoter：mouse derived (MGI:97821). PV is calcium-binding protein.
(2) tetracycline transactivator (tTA)：Fusion protein of tetracycline repressor (Escherichia coli-derived) and VP16 (Herpes simplex virus-derived). In the absence of Doxycycline (Dox), tTA binds to tetracycline-responsive element (TRE) and expression of TRE-controlled genes can be induced.
Vector：pBlueScript (Stratagene), pBACe3.6 (CHORI)
Strain characteristics
tetracycline transactivator(tTA) is expressed in Parvalbumin-positive cells (neurons). In the absence of Dox, tTA binds to TRE and expression of TRE-controlled genes can be induced only in the Parvalbumin-positive cells.
Expression of TRE-controlled genes can be repressed by Dox. (Tet-Off system)
Breeding Conditions
Maintained by mating between heterozygous rats.
Genotyping
Genomic PCR analysis
Forward primer : 5'-ATGTCTAGAC TGGACAAGAG-3'
Reverse primer : 5'-TTACCCGGGG AGCATGTCAA-3'
PCR condition；95?C-5 min, 35 cylcles of (94?C-1 min, 56?C-1 min, 72?C-2 min), 72?C-7 min
PCR product；約750 bp