| NBRP Rat No: 0798 |
Strain name: LE-Tg(Pvalb-tTA)15Hio |
Commmon Name: LE-Tg(mPvalb-tTA)Hio |
Rat Genome Database |
| Principal Investigator: |
Hiroyuki Hioki Graduate School of Medicine, Kyoto University Yoshidakonoe-cho, Sakyo-ku 606-8501 Kyoto JAPAN |
| Tel: 075-753-4336 Fax: 075-753-4340 |
Email: hioki@mbs.med.kyoto-u.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
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| Inbred Generations |
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| Usage Restrictions |
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
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| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
tTA: tetracycline transactivator, Pvalb: Parvalbumin |
| Origin |
background strain：Long-Evans rat（Iar:Long-Evans）
Transgene：
(1) Parvalbumin (Pvalb) promoter：mouse derived (MGI:97821). PV is calcium-binding protein.
(2) tetracycline transactivator (tTA)：Fusion protein of tetracycline repressor (Escherichia coli-derived) and VP16 (Herpes simplex virus-derived). In the absence of Doxycycline (Dox), tTA binds to tetracycline-responsive element (TRE) and expression of TRE-controlled genes can be induced.
Vector：pBlueScript (Stratagene), pBACe3.6 (CHORI)
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| Strain characteristics |
tetracycline transactivator(tTA) is expressed in Parvalbumin-positive cells (neurons). In the absence of Dox, tTA binds to TRE and expression of TRE-controlled genes can be induced only in the Parvalbumin-positive cells.
Expression of TRE-controlled genes can be repressed by Dox. (Tet-Off system) |
| Breeding Conditions |
Maintained by mating between heterozygous rats. |
| Genotyping |
Genomic PCR analysis
Forward primer : 5'-ATGTCTAGAC TGGACAAGAG-3'
Reverse primer : 5'-TTACCCGGGG AGCATGTCAA-3'
PCR condition；95?C-5 min, 35 cylcles of (94?C-1 min, 56?C-1 min, 72?C-2 min), 72?C-7 min
PCR product；約750 bp
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| References |
unpublished |
| Additional strain information |
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