Strain information
NBRP Rat No: 0801
Strain name
: F344.AR-
Ednrb
sl
/Hkv
Commmon Name: F344-
Ednrb
sl
Rat Genome Database
Principal Investigator:
Takashi Agui Graduate School of Veterinary Medicine Nishi 9 Kita 18 jou Kita-ku, Sapporo 060-0818 Hokkaido Japan
Tel: 011-706-5106 Fax: 011-706-5106
Email: agui@vetmed.hokudai.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白色
Inbred Generations
N10+?
Usage Restrictions
Users should be required an approval form from dipositor for 5 years after deposition.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
endothelin receptor type B: Ednrb
Origin
This strain derived from AR (aganglionosis) rat found by Dr. Ikadai in 1973. This congenic strain was established by backcrossing (over 10 generations) of AR rat to F344/Nslc.
Strain characteristics
Homozygous rats shows auditory disorder (congenital deafness). 40% of homozygous rats dies of megacolon and this mortality is lower than that of AGH rats (100%). Heterozygous (sl/+) rats don't show any phenotypes.
Breeding Conditions
maintained in heterozygous condition: A part of homozygous rats dies of megacolon.
Genotyping
Forward Primer: CCTCCTGGACTAGAGGTTCC, Reverse Primer: ACGACTTAGAAAGCTACACT Product size wild-type：511 bp, mutant：210 bp
References
Dang et al. QTL analysis identifies a modifier locus of aganglionosis in the rat model of Hirschsprung disease carrying Ednrb(sl) mutations. PLoS ONE (2011) vol. 6 (11) pp. e27902
Dang et al. Genetic background strongly modifies the severity of symptoms of Hirschsprung disease, but not hearing loss in rats carrying Ednrb(sl) mutations. PLoS ONE (2011) vol. 6 (9) pp. e24086
Dang et al. Lymphopenia in Ednrb-deficient rat was strongly modified by genetic background. Biomed Res (2012) vol. 33 (4) pp. 249-53
Additional strain information