Strain information  NBRP Rat No: 0825  Strain name: W-Tomm22em1Kyo  Commmon Name: HisTom22, W-Tg(His-TOMM22)Tend Rat Genome Database Principal Investigator:  Toshiya Endo  Faculty of Life Sciences, KYOTO SANGYO UNIVERSITY       Motoyama, Kamigamo, Kita-ku, Kyoto    603-8555 Kyoto     Japan Tel: 075-705-1508    Fax: 075-705-1508 Email: tendo@cc.kyoto-su.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  白 Inbred Generations   Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others 構造生物学／細胞生物学  Control Strain  Marker Strain Gene Affected Tomm22: translocase of outer mitochondrial membrane 22 homolog (yeast) Origin This rat strain was established by using the CRISPR-Cas9 method to knock in a 10x histidine tag and a TEV protease recognition site that cleaves the histidine tag at the N-terminus of Tomm22, which forms the mitochondrial translocator complex. It was generated in 2015 at the Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University using Jcl:Wistar as the parent strain. Strain characteristics Homozygous (His-TOMM22 / His-TOMM22 ) is embryonic lethal. Breeding Conditions Can be maintained by crossing heterozygous (His-TOMM22/+) rats Genotyping Genotyping can be determined by PCR using the following primers: Tomm22-SmallF: GAGCTGGAGGAGCTGTCAAC, Tomm22-SmallR: TCCTCCTCAGCTTTCTCTGC Product size: wild-type Tomm22: 210 bp, His-Tomm22: 273 bp References Additional strain information