| NBRP Rat No: 0842 |
Strain name: LE-Tg(Chat-tdTomato)3Yyan |
Commmon Name: ChAT-tdTomat transgenic rats, Long Evans-Tg (ChAT-tdTomato) |
Rat Genome Database |
| Principal Investigator: |
Yuchio Yanagawa Gunma University Graduate School of Medicine 3-39-22 Showa-machi, Maebashi 371-8511 Gunma Japan |
| Tel: 027-220-8040 Fax: 027-220-8046 |
Email: yuchio@gunma-u.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
white with black hood |
| Inbred Generations |
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| Usage Restrictions |
1: Recipient agrees to describe the following in acknowledgement in all written publications as related to the Bioresource. “ChAT-tdTomato transgenic rats were generated by Drs. R. Kaneko, Y. Yanagawa and Y. Saito in Gunma University Graduate School of Medicine, using tdTomato cDNA provided by Dr. R. Tsien.”
2: Recipient agrees to send the reprints of written publications as related to the Bioresource to Drs. R. Kaneko, Y. Yanagawa and Y. Saito, when they are published.
(Address)
Ryosuke Kaneko
Bioresource Center
Gunma University Graduate School of Medicine
3-39-22 Showa-machi, Maebashi 371-8511, Japan
E-mail: rkaneko@gunma-u.ac.jp
Yuchio Yanagawa
Department of Genetic and Behavioral Neuroscience
Gunma University Graduate School of Medicine
3-39-22 Showa-machi, Maebashi 371-8511, Japan
E-mail: yuchio@gunma-u.ac.jp
Yasuhiko Saito
Department of Neurophysiology,
Nara Medical University
Kashihara 634-8521, Japan
E-mail: syasu@naramed-u.ac.jp
3: The Bioresource should not be redistributed without Dr. Yanagawa’s permission.
4: The Bioresource should not be used for any commercial purpose.
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| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
choline acetyltransferase (Chat) マウス由来ゲノムDNA；tdTomato サンゴ由来 cDNA；beta-globin polyA配列 ウサギ由来ゲノムDNA；FRT配列 酵母由来ゲノムDNA |
| Origin |
Chat-tdToamto-polyA-FRT sequence was inserted into fertilized egg of Long Evans strain (Japan SLC). vector: BAC clone (RP23-246B12) from a C57BL/6J mouse genomic BAC library (BACPAC Resource Center, Oakland, CA, USA)
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| Strain characteristics |
tdTomato protein is expressed in cholinergic neurons. |
| Breeding Conditions |
mating Tg rats with wild-type Long Evans rats |
| Genotyping |
pCXpA-F: 5’-TCCTCCTCTCCTGACTACTC-3’
ChAT+293R: 5’-GACTACTGGAACCAGAGTTG-3’
PCR product: 395 bp |
| References |
Zhang Y, Kaneko R, Yanagawa Y, Saito Y.
The vestibulo- and preposito-cerebellar cholinergic neurons of a ChAT-tdTomato transgenic rat exhibit heterogeneous firing properties and the expression of various neurotransmitter receptors.
Eur J Neurosci. 39, 1294-313, 2014
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| Additional strain information |
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