Strain information
 NBRP Rat No: 0849  Strain name: ExHC.BN-(D14Got74-D14Rat132)/Kyu  Commmon Name: Ex.BN-Smek2 Rat Genome Database
Principal Investigator:  Masao Sato  Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University       6-10-1 Hakozaki, Higashi-ku, Fukuoka     812-8581 Fukuoka     Japan
Tel: 092-642-3004    Fax: 092-642-3004 Email: masaos@agr.kyushu-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ?s_Metabolism=1 ?s_Metabolism=1
Coat Color  albino(c)
Inbred Generations  F21
Usage Restrictions   The recipient is requested to cite the literature designated by the DEPOSITOR.
M. Asahina, M. Sato , K. Imaizumi, Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic rats, Journal of Lipid Research, 46, 10, 2289-2294, 46: 2289-2294, 2005.10.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected
Origin This congenic strain was established by introducing a segment of chromosome14 (D14Got74-D14Rat132 including Smek2 gene) from Brown Norway(BN)/seac strain into ExHC/Seac strain.
Strain characteristics Unlike in the case of ExHC/Seac strain, dietary hypercholesteremia is not induced by feeding of 1% cholesterol diet (for 2-4 weeks).
Breeding Conditions Sib mating. Good breeding perfprmance.
Genotyping Congenic area is detected by microsatellite markers.
The sizes of PCR products of D14Rat, D14Rat17, D14Rat26, D14Rat43, D14Rat18, D14Rat94, D14Rat95 are same as ExHC/Seac. The sizes of PCR products of D14Got74, D14Got76, D14mco4, D14Rat132 are same as BN/Seac.
PCR condition: 95℃ 2min, (94℃ 30sec, 52.5℃ 30 sec, 72℃ 30 sec)×38 cycles, 72℃ 5 min
References Asahina M, Sato M, Imaizumi K.

Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic rats.

J Lipid Res. 2005 Oct;46(10):2289-94.

Asahina M, Haruyama W, Ichida Y, Sakamoto M, Sato M, Imaizumi K.

Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats.

J Lipid Res. 2009 Jan;50(1):41-6.
Additional strain information