Strain information
 NBRP Rat No: 0854  Strain name: W-Ift140em1Kyo  Commmon Name: Ift140 KOラット em1 Rat Genome Database
Principal Investigator:  Akira Sato  The Institute of Environmental Toxicology           303-0043      Japan
Tel: 0297-27-4537    Fax: 0297-27-4519 Email:
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  N2
Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested.
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
1.The users should not publishany research results obtained by usingthis resource without consent of the depositors until the depositors will publish the original research paper regarding the development of this resource.
2.Any research papers published by the users should refer to the depositor's originalresearch paper in the clause 1.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Ift140 (intraflagellar transport 140)
Origin This strain was established by CRISPR/Cas9 system at Kyoto University and introduced to the Institute of Environmental Toxicology. Background strain: Jcl:Wistar. This line 1 (em1) has 2-bp insertion (c.23_24insGG) in the exon 1 of Ift140 gene.
Strain characteristics Under analysis
Breeding Conditions Maintained by mating between heterozygous rats. Homozygous rats are presumed to be embyonic lethal.
Genotyping Forward primers are Ift140D_W_F1 (ATGGCACTGTATTTTGACCACCGAA), Ift140D_INS_F1 (ATGGCACTGTATTTTGACCACCGGG), and Ift140D_DEL_F1 (ATGGCACTGTATTTTGACCACCATC). Revers primers is Ift140_Ex1_1.0R (CTCCTGGCCTCTATGACTCG). PCR condition: 94℃/10 min;(94℃/30 sec,55℃/30 sec,72℃/30 sec)30 cycles (2xAmpdirect Plus, BIOTAQ HS DNA Polymerase). Productsizes: wild (228 bp), em1 (230 bp), and em2 (226 bp).
References 未発表、投稿準備中
Additional strain information