NBRP Rat No: 0854
Commmon Name: Ift140 KOラット em1
Rat Genome Database
Akira Sato The Institute of Environmental Toxicology 303-0043 Japan
Tel: 0297-27-4537 Fax: 0297-27-4519
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Research Area Others
Ift140 (intraflagellar transport 140)
This strain was established by CRISPR/Cas9 system at Kyoto University and introduced to the Institute of Environmental Toxicology. Background strain: Jcl:Wistar. This line 1 (em1) has 2-bp insertion (c.23_24insGG) in the exon 1 of Ift140 gene.
Maintained by mating between heterozygous rats. Homozygous rats are presumed to be embyonic lethal.
Forward primers are Ift140D_W_F1 (ATGGCACTGTATTTTGACCACCGAA), Ift140D_INS_F1 (ATGGCACTGTATTTTGACCACCGGG), and Ift140D_DEL_F1 (ATGGCACTGTATTTTGACCACCATC). Revers primers is Ift140_Ex1_1.0R (CTCCTGGCCTCTATGACTCG). PCR condition: 94℃/10 min；（94℃/30 sec，55℃/30 sec，72℃/30 sec）30 cycles (2xAmpdirect Plus, BIOTAQ HS DNA Polymerase). Productsizes: wild (228 bp), em1 (230 bp), and em2 (226 bp).
Additional strain information