Strain information
NBRP Rat No: 0854
Strain name
: W-
Ift140
em1Kyo
Commmon Name: Ift140 KOラット em1
Rat Genome Database
Principal Investigator:
Akira Sato The Institute of Environmental Toxicology 303-0043 Japan
Tel: 0297-27-4537 Fax: 0297-27-4519
Email: a.satoh@iet.or.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
アルビノ
Inbred Generations
N2
Usage Restrictions
In publishing, an acknowledgment to the DEPOSITOR is requested.
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
1.The users should not publishany research results obtained by usingthis resource without consent of the depositors until the depositors will publish the original research paper regarding the development of this resource.
2.Any research papers published by the users should refer to the depositor's originalresearch paper in the clause 1.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Ift140 (intraflagellar transport 140)
Origin
This strain was established by CRISPR/Cas9 system at Kyoto University and introduced to the Institute of Environmental Toxicology. Background strain: Jcl:Wistar. This line 1 (em1) has 2-bp insertion (c.23_24insGG) in the exon 1 of Ift140 gene.
Strain characteristics
Under analysis
Breeding Conditions
Maintained by mating between heterozygous rats. Homozygous rats are presumed to be embyonic lethal.
Genotyping
Forward primers are Ift140D_W_F1 (ATGGCACTGTATTTTGACCACCGAA), Ift140D_INS_F1 (ATGGCACTGTATTTTGACCACCGGG), and Ift140D_DEL_F1 (ATGGCACTGTATTTTGACCACCATC). Revers primers is Ift140_Ex1_1.0R (CTCCTGGCCTCTATGACTCG). PCR condition: 94℃/10 min；（94℃/30 sec，55℃/30 sec，72℃/30 sec）30 cycles (2xAmpdirect Plus, BIOTAQ HS DNA Polymerase). Productsizes: wild (228 bp), em1 (230 bp), and em2 (226 bp).
References
未発表、投稿準備中
Additional strain information