Strain information
 NBRP Rat No: 0868  Strain name: WKY.Cg-clest/Iet  Commmon Name: Cleft lip/palate, Ectrodactyly and Stunted Growth, CLEST, WKY.Cg-clest Rat Genome Database
Principal Investigator:  Akira Sato  The Institute of Environmental Toxicology           303-0043      Japan
Tel: 0297-27-4537    Fax: 0297-27-4519 Email: a.satoh@iet.or.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  N6
Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
1.The users should not publishany research results obtained by usingthis resource without consent of the depositors until the depositors will publish the original research paper regarding the development of this resource.
2.Any research papers published by the users should refer to the depositor's originalresearch paper in the clause 1.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected unknown
Origin When the depositor (The Institute of Environmental Toxicology ) conducted mating experiment using male Crl:CD(SD) rats, the offsprings of backcrossed-generation showed multiple malformations such as ectrodactylism, cleft palate or lip in 2013. These phenotype might be inherited in an autosomal recessive pattern. Therefore the congenic strain was established using WKY strain as the recipient strain.
Strain characteristics Homozygous rats have very small body and develop external malformations such as ectrodactylism, cleft palate or lip, brachyury, and aproctia. These homozygous pups die soon after birth. In addition, defect of the kidney or small kidney and cardiovascular mulformations including overriding aorta are also observed. In skeletal system, severe malforamations of the axial skeleton such as rib fusion and abnormal arrangement of vertebral bodies: these phenotypes are extremely similar to those of the mouse molel for Spondylocostal dysostosis (SCDO). However, during establishment of congenic strain, the occurrence rate of malformated offsprings was significantly diminished and it is difficult to observe these phenotype now. Additional experiment revealed that homozygous malformated embryos with hematoma can be detected from embryonic day 12 and most of them die at embryonic day 13. The causative gene has not yet been identified: as a result of linkage analysis, this mutation might be located the area of D10Rat148-D10Got216.
Breeding Conditions Maintained in heterozygous condition (mating heterozygous male rats with female WKY rats).
Genotyping Check the genotype of 3 loci; D10Rat148（100 bp＜SD＜WKY=155bp），D10Rat55（100 bp＜WKY＜SD＜200bp) and D10Got216（100 bp＜SD＜WKY＜200bp). Heterozygous rats have SD-derived marker alleles. Please contact the depositor for detailed information. Genotyping result
References unpublished
Additional strain information