Rat Strains

Principal Investigator:

Yumiko Yoshimura National Institute for Physiological Science 38 Nishigonaka Myodaiji 444-8585 Okazaki, Aichi Japan

Preservation Status:

Embryo Sperm Living Animals

Coat Color

白黒

Inbred Generations

F?+?+0 (17,02,16)

Usage Restrictions

1.The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.Recipient agrees to cite the follwing paper in all written publications as related to the Material.
Umematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi N, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex18,315-330,2008.
3.Recipient agree to describr the following in acknowledgement in all written publications as related to the Materials.
'VGAT-Venus transgenic rats were generated by Drs.Y.Yanagawa,M.Hirabayashi and Y.Kawaguchi in National Insititute for physiological Sciences, Okazaki,Japan,usingpCS-2Venus provided by Dr.A.Miyawaki.'
4.Recipient agrees to send the reprints of written publications as related to the Materials to Drs.Tasuo Kawaguchi and Yuchio Yanagawa, when they are published.
(Address)
Yuchio Yanagawa
Department of Genetic and Beavioral Neuroscience
Gunma University Graduate School of Medicine
3-39-22 Showa-machi,Maebashi 371-8511,Japan
Email:yanagawa@med.gunma-u.ac.jp

Yasuo Kawaguchi
Division of Cerebral Circuitry
National Institute for Physiological Sciences
Myodaiji,Okazaki 444-8585,Japan
Email:yasuo@nips.ac.jp

Yumiko Yoshimura
Division of Visual Information Processing
National Institute for Physiological Sciences
Myodaiji,Okazaki 444-8585,Japan
Email:yumikoy@nips.ac.jp

5.The Venus gene incorparated into VGAT-Venus rats supplied by Dr.Atsushi Miyawaki at RIKEN,Japan.The following is needed according to the Agreement.

Dr.Atsushi Miyawaki
Laboratory for Cell Function Dynamics
RIKEN Brain Science Institute
2-1 Hirosawa,Wako City Saitama,351-0198,Japan
Email:matsushi@brain.riken.jp

4-1:RECIPIENT agrees to submit the intended publication to Dr.Atsushi Miyawaki prior to the submission date.
4-2:RECIPIENT agrees to acknowledge Dr.Atsushi Miyawaki in all oral presentations and written publications as related to the MATERIAL. For example,“Venus was developed by Dr.Atsushi Miyawaki at RIKEN, Wako,Japan.”
4-3:RECIPIENT agrees to send the reprints in relation to all oral presentaions or written publications as related to the Materials to Dr.Atsushi Miyawaki.

Genetic Status

Comercial Availability

Research Category

Gene Affected

Slc32a1: solute carrier family 32 member 1, YFP* (Venus): yellow fluorescent protein variant

Origin

The original strain, W-Tg(Slc32a1-YFP*)1Yyan (NBRP No.0554 rat), was established at National Institute for Physiological Sciences in 2004, thereafter introduced to Gunma University in 2006 （Uematsu et al. Cerebral Cortex (2008). This original strain rats were was crossed with Long-Evans rat (Iar:Long-Evans) for 10 generations to establish the congenic strain.

Strain characteristics

Inhibitory neurons are labeled with Venus fluorescence (YFP*) under the control of mouse Slc32a1（VGAT, vesicular GABA transporter）promoter. The original strain (W-Tg(Slc32a1-YFP*)1Yyan) was crossed with Long-Evans rat (Iar:Long-Evans) for 10 generations to establish the congenic strain.

Breeding Conditions

mating between hemizygous Tg rat and wild-type (Long-Evans) rat

Genotyping

Genotyping protocol for Slc32a1-YFP*

References

Degro CE, Kulik A, Booker SA, Vida I.
Compartmental distribution of GABAB receptor-mediated currents along the somatodendritic axis of hippocampal principal cells.
Front Synaptic Neurosci. 2015 Mar 23;7:6.

Ueta Y, Otsuka T, Morishima M, Ushimaru M, Kawaguchi Y.
Multiple layer 5 pyramidal cell subtypes relay cortical feedback from secondary to primary motor areas in rats.
Cereb Cortex. 2014 Sep;24(9):2362-76.

Otsuka T, Kawaguchi Y.
Common excitatory synaptic inputs to electrically connected cortical fast-spiking cell networks.
J Neurophysiol. 2013 Aug;110(4):795-806.

*Uematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi M, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex 18, 315-330, 2008.

Additional strain information