Strain information
 NBRP Rat No: 0863  Strain name: W;Cg-Acrtm1Osb  Commmon Name: Acr KO, Acr {tm1 Osb} Rat Genome Database
Principal Investigator:  Masahito Ikawa  Osaka University       3-1 Yamadaoka    565-0871 Suita-shi Osaka     JAPAN
Tel: 06-6879-8375    Fax: 06-6879-8376 Email: mta-egr@biken.osaka-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  albino
Inbred Generations  情報なし
Usage Restrictions  The RECIPIENT of BIOLOGICAL RESOURCE shall obtaina prior written consent use of it from the DEPOSITOR.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following Literature(s) designated by the DEPOSITOR is requested.
The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Acr (acrosin)
Origin After homologous recombination using ES cells derived from hybrid (mix) breed of Slc:Wistar (female) and F344/NSlc (male), Acr KO strain was established by GLT at Osaka University. The genomic region including exon 1 to exon 3 is replaced by Neo resistance gene. After mating with Slc:Wistar, this strain is maintained by sib mating or with Slc:Wistar rats.
Strain characteristics When Acr homozygous KO rats are used for mating, the litter size is significantlly reduced. In female rats, no specific phenotype is observed.
Breeding Conditions Maintained by mating betweeen heterozygous or homozygous females and heterozygous males.
Genotyping PCR reaction mixtures tube 1 (total 15 μl) : DW 2.1 μl, 2×Buffer 7.5 μl, 2mM dNTP 3μl, primer #1 50pmol/μl 0.1 μl, Primer #2 50pmol/μl 0.1 μl, Primer #3 50pmol/μl 0.1 μl, KOD Fx polymerase (TOYOBO) 0.1 μl, DNA 2 μl. Cycling condition : 94 ℃ 2 min, (94 ℃ 0.5 min, 65 ℃ 0.5 min, 72 ℃ 1 min) x 40 cycles, 72 ℃ 2 min. primer #1 name rAcr-Fw (23mer) 5’-TGCTACGTGACTGGGTGGGGATA-3’, primer #2 name rAcr-Rv (23mer) 5’-CGCCTTCTGACAACCTGACACCA-3’, primer #3 name Neo-Fw (22mer) 5’-CAGCCTCTGAGCCCAGAAAGCG-3’. Product size: primer #1 ⇔ primer #2 約250 bp (wild), primer #2 ⇔ primer #3 約500 bp (mutant)
References Isotani, A., Matsumura, T., Ogawa, M., Tanaka, T., Yamagata, K., Ikawa, M., and Okabe, M.
A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.
Biol Reprod. 2017 Jul 1;97(1):61-68.
Additional strain information