Strain information
NBRP Rat No: 0883
Strain name
: F344-
Il2rg
em1Iexas
Commmon Name: X-SCID, F344-Il2rgem1Iexas
Rat Genome Database
Principal Investigator:
Tomoji Mashimo The University of Tokyo, The Institute of Medical Science 4-6-1 Shirokanedai-Minatoku-Tokyoto 108-8639 Japan
Tel: 03-6409-2489 Fax:
Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
albino
Inbred Generations
N3
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
interleukin 2 receptor gamma (Il2rg)
Origin
【This strain is provided by the University of Tokyo (cooperative facility)】 This strain was established by CRISPR/Cas9 targeting rat Il2rg gene using electroporation. background strain: F344/Jcl. gRNA: CCAACCTCACTATGCACTATAGG (PAM sequence: CCA). Cas9: mRNA transcripted from T7-NLS hCas9-pA (RDB13130). Off target analysis has not yet been conducted.
Strain characteristics
This strain shows severe combined immunodeficiency (SCID) caused by 5-bp deletion of Il2rg gene. This strain grows normally under SPF condition.
Breeding Conditions
mating between hemizygous male and homozygous female rats
Genotyping
PCR: primers (TTGCTGACTTCTATGGACCTTAAA and TTCATCTGGTCTGAACTGATAACTTAT). product size: wild 292 bp and mutant 287 bp
References
Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T.
Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases.
PLoS One. 2010 Jan 25;5(1):e8870.
Additional strain information