Strain information
NBRP Rat No: 0898
Strain name
: SD;DA-Tg(Thy1-APP*)12Dspb
Commmon Name: App(Swe/Ind)-Tg12 Dspb
Rat Genome Database
Principal Investigator:
kenryo Furushima 564-0053
Tel: 06-6337-5897 Fax: 06-6337-6020
Email: kenryo-furushima@bio.ds-pharma.co.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白
Inbred Generations
F1+2
Usage Restrictions
This BIOLOGICAL RESOURCE (BR) is produced using a patent "rat embryonic stem cells" owned by Sumitomo Chemical Co.,Ltd(SC).This rat will be made available subject to the following conditions.(a)A recipient of BR shall inform SC of his/her purpose to use the BR, regardless of whether it is commercial or non-commercial,for SC's prior written approval/consent. Contact: YASUHIKO TAKAHASHI<takahashiy15@sc.sumitomo-chem.co.jp>(b)A recipient of BR shall neither distribute nor transfer the BR to third parties.(c)In the event that a recipient publishes his/her research results obtained by the use BR, he/she shall provide an acknowledgement to a DEPOSITOR.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Thy1-hApp
Origin
Mutant human amyloid beta (APP*) combined Thy1 promoter which is specifically expressed in neurons, was induced to ES cells from DA strain rats, and chimera rats obtained from the ES cells. The F1 rats were backcrossed with Crl:CD (SD) strain rats and F3 rats was deposited.
Strain characteristics
The tendency of dementia-like behavior (declining object perception) is observed in a novel object recognition test. The expression levels of mutant human APP mRNA are difficult among Lines (12＞1＞14＞6).
Breeding Conditions
Mating the transgenic rats with wild type rats and maintaining as heterozygous rats.
Genotyping
Decide the transgenic rat by PCR using the following primers. If approximately 9 kb fragments are amplified from the rat genome, the rat is decided as transgenic rat. Primer: TA-F 5’-GAATTCAGAGACCGGGAACCAAACTAG-3’ Primer: TA-R 5’- GAGAGAGAATAAGGACAGTGAACGAGTGG-3’
References
Additional strain information