Strain information
NBRP Rat No: 0821
Strain name
: F344-
Hcn1
em1Kyo
Commmon Name: Hcn1 KO (Line84) , F344-
Hcn1
em84Kyo
Rat Genome Database
Principal Investigator:
Takashi Kuramoto Graduate School of Medicine, Kyoto University Yoshidakonoe-cho, Sakyo-ku 606-8501 Kyoto Japan
Tel: 075-753-4494 Fax: 075-753-4409
Email: kuramot@anim.med.kyoto-u.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白色
Inbred Generations
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
hyperpolarization-activated cyclic nucleotide-gated 1 channel (Hcn1)
Origin
TALEN (Left:ttcagＡＡＴＧＡＴＴＣＡＴＧＧＧ, Right:ＡＣＧＣＡＣＴＣＴＴＣＡＡＡＧＣＴＡ）targeting the exon4 of hyperpolarization-activated cyclic nucleotide-gated 1 channel (Hcn1) gene was designed and mRNA coding these TALEN was microinjected into F344/NSlc embyo.
Strain characteristics
A 7-bp deletion in the exon4 of Hcn1 gene: as a result of frameshift mutation, stop codon is produced. Decreased expression levels of Hcn1 gene and HCN1 protein.
Breeding Conditions
maintained by crossing between homozygous and homozygous, or homozygous and heterozygous rats
Genotyping
rHcn1-e4-KO-F：ttccaaccatttgcctacaa rHcn1-e4-KO-R：gccataaccaatgcacagc PRODUCT SIZE：wild 239, KO 232
References
Additional strain information