NBRP Rat No: 0903
Commmon Name: TRPA1KOrat
Rat Genome Database
Kazuaki Ohara Kirin Company,Limited 1-13-5 fukuura-kanazawaku, 236-0004
Tel: 080-2196-6648 Fax: 045-788-4042
In publishing, the reserach results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Ohara et al.,"B-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system." Scirep. 2017 Nov 17; 7(1):15785
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Research Area Others
-deleted Wistar (Crl:WI) strain was generated by using Zinc Finger Nuclease at Kirin Company, Limited in 2013. Exon 22-24, which form ion channel pore required for the activation in Trpa1 gene, was deleted.
Noticeable phenotype is not observed under normal breeding. Intracellular calcium elevation treated by TRPA1-activating agent is not occurred under the calcium imaging using dorsal root ganglion neurons.
Determine by genomic PCR. Primer: Mt-F; CCTGATTTGCTCTGGCACA, Int24CellF; TGGGACTGTGTGGTGCT, Int24IgdelR; CCACAGTTCACCACCTTTCA PCR enzyme:KOD FX (Toyobo) Component Volume: template 1.0 μl Mt-F (10 μM) 0.75 μl Int24 lgdel R (10 μM) 0.75 μl Int24 Cel1 F (10 μM) 0.75 μl 2 x FX buffer 12.5 μl 2.0 mM dNTPs each 5.0 μl KOD FX 0.5 μl ddw 3.75 μl Total 25.0 μl Cycling condition (35 cycles): Predenature : 94.0 ℃ 2 min. Denature : 98.0 ℃ 10 sec. Annealing : 62.5 ℃ 30 sec. Extention : 68.0 ℃ 30 sec. Final extension : 68.0 ℃ 30 sec. Hold : 4.0 ℃ Size of PCR products: Mutant 300bp Wild 563 bp
β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system.
Ohara K, Fukuda T, Ishida Y, Takahashi C, Ohya R, Katayama M, Uchida K, Tominaga M, Nagai K.
Sci Rep. 2017 Nov 17;7(1):15785. doi: 10.1038/s41598-017-16150-6.
Additional strain information