Strain information
NBRP Rat No: 0887
Strain name
: W-
Phf24
em11Iexas
Commmon Name: Crlj:WI-Phf24 Δ128, WI-Phf24 em1Iexas
Rat Genome Database
Principal Investigator:
Tadao Serikawa Kyoto Disease Model Institute The Kyoto Technoscience Center, Room No. 22, 14 Yoshida-kawara-machi, Sakyo-ku, Kyoto 606-8305 Kyoto Japan
Tel: 075-708-2902 Fax: 075-708-2935
Email: serikawa@anim.med.kyoto-u.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
アルビノ
Inbred Generations
F2-3世代 (2017.11.12)
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Phf24 (旧名称Rgd1559864, Kiaa1045)
Origin
This strain was establishe by CRISPR-Cas9 system at Osaka University. Target sequence is CCATGGGGGTGTTGATGTCCAAG (CCA is PAM sequence). Back ground strain is Crlj:Wistar (WI). This strain is line No. 11 and has a 128-bp deletion in Phf24 gene. Off-target effects (214 candidate region) have not yet been examined.
Strain characteristics
No significant abnormalities by gross examination
Breeding Conditions
maintained in homozygous condition
Genotyping
Primers are KIAA-1045 Small F: CCTGTGTAGGGTGCTGAACA and KIAA-1045 Small R: CGCTTCCTTCTCCTCCTGTA. The product size of wild type rat is 385bp and 257 bp (CCAGAGTCTTC-128-CCATGGGGGTGTTGATGTCCAAGCGGCA) band can be detected in KO rat.
References
論文未発表
Additional strain information