Strain information
 NBRP Rat No: 0889  Strain name: W-Phf24em6(EGFP)Iexas  Commmon Name: Crlj:WI-Phf24-Gfp KI, WI-Phf24 em3Iexas Rat Genome Database
Principal Investigator:  Tadao Serikawa  Kyoto Disease Model Institute       The Kyoto Technoscience Center, Room No. 22, 14 Yoshida-kawara-machi, Sakyo-ku, Kyoto    606-8305 Kyoto     Japan
Tel: 075-708-2902    Fax: 075-708-2935 Email: serikawa@anim.med.kyoto-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  F2-3世代 (2017.11.12)
Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Phf24 (旧名称Rgd1559864, Kiaa1045)
Origin This strain (line No. 6) was establishe by CRISPR-Cas9 system at Osaka University and EGFP sequence was knock-in in Phf24 gene. Back ground strain is Crlj:Wistar (WI). Founder rats were crossed with F344/NSlc, and this strain was maintained by crossing with F344/NSlc (F2 or F3 generation, November 2017). At the point of sperm cryopreservation, this strain is not "congenic" strain (backcross generation was <5 generations), but background is mix of Crlj:Wistar and F344/NSlc.
Strain characteristics No significant abnormalities by gross examination
Breeding Conditions crossing with F344/NSlc
Genotyping KIAA-1045 Small F: CCTGTGTAGGGTGCTGAACA KIAA-1045 Small R: CGCTTCCTTCTCCTCCTGTA EGFP primers (upstream) EGFP up Small F: CGTAAACGGCCACAAGTTCA EGFP up Small R: GCATGGCGGACTTGAAGAAG EGFP orimers（downstream） EGFP down Small F: AACTTCAAGACCCGCCACAA EGFP down Small R: GTCCATGCCGAGAGTGATCC joint, upstream KIAA-1045 Small F: CCTGTGTAGGGTGCTGAACA EGFP up Small R: GCATGGCGGACTTGAAGAAG joint, downstream EGFP down Small F: AACTTCAAGACCCGCCACAA KIAA-1045 Small R: CGCTTCCTTCTCCTCCTGTA Results: KIAA-1045-Small-F + KIAA-1045-Small-R (385bp), EGFP-up-Small-F + EGFP-up-Small-R (203bp), 　 EGFP-down-Small-F + EGFP-down-Small-R (213bp), 　 KIAA-1045-Small-F + EGFP-up-Small-R (470bp), GFP-down-Small-F + Phf24-R (958bp)
References 論文未発表
Additional strain information