Strain information  NBRP Rat No: 0914  Strain name: WIC-Cspg4em3Kyst  Commmon Name: CSPG4 (+2) Rat Genome Database Principal Investigator:  Keitaro Yamanouchi  The University of Tokyo        1-1-1, Yayoi, Bunkyo-ku,    113-8657 Tokyo     Japan Tel: 03-5841-5387    Fax: 03-5841-8017 Email: akeita@mail.ecc.u-tokyo.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  白色 Inbred Generations   Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected コンドロイチン硫酸プロテオグリカン4（CSPG4） Origin By CRISPR/Cas9 system, mutation was introduced in Cspg4 gene of Wistar-Imamichi rat (Iar: Wistar-Imamichi, Institute for Animal Reproduction). 2 bp insertion on Exon1. Strain characteristics Analyzing Breeding Conditions Natural mating Genotyping To distinguish between wild type and Cspg4 knockout (KO) rat, run PCR followed by treat the DNA fragments by using the restriction enzyme. Primer F: CTTAGAGACATTGCCCCAAATCTT Primer R: GATACCCGGAGTTCTCATAAACAGA PCR（DNA polymerasae: KOD FX Neo） 94℃ 5 min, x 1 cycle 98℃ 10 sec, 58℃ 30 sec, 68℃ 40 sec x 38 cycles Cut DNA fragments by Mwo1 (restriction enzyme). Wild type: one DNA band is observed around 340 bp. Cspg4 KO (hetero): two DNA bands are observed around 200 bp and 340 bp. Cspg4 KO (homo): one DNA band is observed around 200 bp. References Additional strain information