Strain information
 NBRP Rat No: 0916  Strain name: W-Tg(Grp-YFP*)14Hskmt  Commmon Name: Wistar-Tg(pGrp-Venus) Rat Genome Database
Principal Investigator:  Hirotaka Sakamoto  Okayama University       130-17 Ushimadochokashino, Setouchi, Okayama    701-4303     
Tel: 0869-34-5210    Fax: 0869-34-5211 Email: hsakamo@okayama-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白色
Inbred Generations  F10
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested:OtiT Takanami K, Ito S, Ueda T, Matsuda KI, kawata M, Soh J, Ukimura O, Sakamoto T, Sakamoto H:Effects of sex steroids on the spinal gastrin-releasing peptide system controlling male sexual function in rats. Endocrinology 159:1886-1896.
In publishing the research resultsto be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgement to the DEPOSITOR is requested.
The following specific terms and conditions are requested by the DEPOSITOR. In this event, the recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE should be used under the following rules.
1)Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting licence agreements with TOHOKU University.
2)All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that OKAYAMA University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOURCES without obligation for Compensation.
3)Either the BILOGICAL RESOURCE or the biological materials
included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4)The BIOLOGICAL RESOURCE should not be redistributed without the permission by OKAYAMA University.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected gastrin-releasing peptide (GRP)-promoter YFP* (Venus): yellow fluorescent protein variant
Origin Background strain: Crlj:WI
This strain was generated by microinjecting Venus, which is inserted at downstream of Grp-promoter (4 kb) to Wistar rat zygotes. The number of inserted gene is approximately 100 copy.
Strain characteristics This transgenic rat for labeling GRP-expressing neuron under the rat gastrin-releasing peptide (GRP)-promoter. In the cell having X-zone of lumbar spinal cord L3-L4 revel, 99% of GRP-expressing neuron are expressed Venus.
Breeding Conditions Mate the heterozygous Tg rat and wild type Wister (Crlj:WI) and distinguish transgenic rats by genotyping the pups.
Genotyping Amplify Venus gene by PCR. Venus primer sequence (forward): 5’-CACCATCTTCTTCAAGGACGAC-3’ (reverse) 5’-ATGATATAGACGTTGTGGCTGTTGT-3’PCR condition 95℃ 15 s, 50℃ 15 s, 72℃ 15 s　35 cycles, 72℃ 5min, Electrophorese the PCR products and distinguish Tg by check the band at 171 bp.
References Oti T, Takanami K, Ito S, Ueda T, Matsuda KI, Kawata M, Soh J, Ukimura O, Sakamoto T, Sakamoto H Effects of sex steroids on the spinal gastrin-releasing peptide system controlling male sexual function in rats. Endocrinology 159: 1886-1896. doi: 10.1210/en.2018-00043.
Additional strain information