NBRP Rat No: 0907 |
Strain name: LE-Gad2em24Yyan |
Commmon Name: GAD65ノックアウトラット, Gad2遺伝子ノックアウトラット(Long-Evans rat) |
Rat Genome Database |
Principal Investigator: |
Yuchio Yanagawa Gunma University Graduate School of Medicine 3-39-22 Showa-machi, Maebashi 371-8511 Gunma Japan |
Tel: 027-220-8040 Fax: 027-220-8046 |
Email: yuchio@gunma-u.ac.jp |
Preservation Status: |
Embryo Sperm Living Animals |
![?s_Development=1]() |
![?s_Development=1]() |
Coat Color |
Black hood |
Inbred Generations |
F3以上 |
Usage Restrictions |
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Genetic Status |
|
Comercial Availability |
|
|
Research Category |
|
Gene Affected |
ラット Gad2遺伝子 |
Origin |
Knockout rats in which the Gad2 gene was disrupted using the TALEN. |
Strain characteristics |
Glutamate decarboxylase (GAD; there are two isoforms, GAD65 and GAD67) is an enzyme that synthesizes the neurotransmitter GABA. In this strain, the Gad2 gene encoding GAD65 was knocked out. In homozygous rats, a high rate of epileptic seizures was observed at 2 to 3 weeks of age. |
Breeding Conditions |
Most of the homozygous rats die as juveniles. This strain is maintained in heterozygotes. |
Genotyping |
Genotyping using PCR. |
References |
Rats deficient in the GAD65 isoform exhibit epilepsy and premature lethality.
Kakizaki T, Ohshiro T, Itakura M, Konno K, Watanabe M, Mushiake H, Yanagawa Y.
FASEB J. 2021 Feb;35(2):e21224.
Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats.
Jiang W, Kakizaki T, Fujihara K, Miyata S, Zhang Y, Suto T, Kato D, Saito S, Shibasaki K, Ishizaki Y, Isoda K, Yokoo H, Obinata H, Hirano T, Miyasaka Y, Mashimo T, Yanagawa Y.
FASEB J. 2022 Feb;36(2):e22123. |
Additional strain information |
|
|