Strain information
 NBRP Rat No: 0907  Strain name: LE-Gad2em24Yyan  Commmon Name: GAD65ノックアウトラット, Gad2遺伝子ノックアウトラット（Long-Evans rat） Rat Genome Database
Principal Investigator:  Yuchio Yanagawa  Gunma University Graduate School of Medicine       3-39-22 Showa-machi, Maebashi     371-8511 Gunma     Japan
Tel: 027-220-8040     Fax: 027-220-8046 Email: yuchio@gunma-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ?s_Development=1 ?s_Development=1
Coat Color  Black hood
Inbred Generations  F3以上
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected ラット　Gad2遺伝子
Origin Knockout rats in which the Gad2 gene was disrupted using the TALEN.
Strain characteristics Glutamate decarboxylase (GAD; there are two isoforms, GAD65 and GAD67) is an enzyme that synthesizes the neurotransmitter GABA. In this strain, the Gad2 gene encoding GAD65 was knocked out. In homozygous rats, a high rate of epileptic seizures was observed at 2 to 3 weeks of age.
Breeding Conditions Most of the homozygous rats die as juveniles. This strain is maintained in heterozygotes.
Genotyping Genotyping using PCR.
References Rats deficient in the GAD65 isoform exhibit epilepsy and premature lethality.
Kakizaki T, Ohshiro T, Itakura M, Konno K, Watanabe M, Mushiake H, Yanagawa Y.
FASEB J. 2021 Feb;35(2):e21224.

Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats.
Jiang W, Kakizaki T, Fujihara K, Miyata S, Zhang Y, Suto T, Kato D, Saito S, Shibasaki K, Ishizaki Y, Isoda K, Yokoo H, Obinata H, Hirano T, Miyasaka Y, Mashimo T, Yanagawa Y.
FASEB J. 2022 Feb;36(2):e22123.
Additional strain information