Strain information
 NBRP Rat No: 0906  Strain name: LE-Gad1em15Yyan  Commmon Name: GAD67ノックアウトラット, Gad1遺伝子ノックアウトラット（Long-Evans rat） Rat Genome Database
Principal Investigator:  Yuchio Yanagawa  Gunma University Graduate School of Medicine       3-39-22 Showa-machi, Maebashi     371-8511 Gunma     Japan
Tel: 027-220-8040     Fax: 027-220-8046 Email: yuchio@gunma-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  Black hood
Inbred Generations  F2~F3
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected ラット　Gad1遺伝子
Origin Knockout rats in which the Gad1 gene was disrupted using the CRISPR/Cas9.
Strain characteristics Glutamate decarboxylase (GAD; there are two isoforms, GAD65 and GAD67) is an enzyme that synthesizes the neurotransmitter GABA. In this strain, the Gad1 gene encoding GAD67 was knocked out. In homozygous rats, low body weight at 3 weeks and impaired spatial learning memory were observed.
Breeding Conditions Some homozygous rats die as juveniles. This strain is maintained in heterozygotes.
Genotyping Genotyping using PCR.
References CRISPR/Cas9-engineered Gad1 elimination in rats leads to complex behavioral changes: implications for schizophrenia.
Fujihara K, Yamada K, Ichitani Y, Kakizaki T, Jiang W, Miyata S, Suto T, Kato D, Saito S, Watanabe M, Kajita Y, Ohshiro T, Mushiake H, Miyasaka Y, Mashimo T, Yasuda H, Yanagawa Y.
Transl Psychiatry. 2020 Dec 8;10(1):426.

Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats.
Jiang W, Kakizaki T, Fujihara K, Miyata S, Zhang Y, Suto T, Kato D, Saito S, Shibasaki K, Ishizaki Y, Isoda K, Yokoo H, Obinata H, Hirano T, Miyasaka Y, Mashimo T, Yanagawa Y.
FASEB J. 2022 Feb;36(2):e22123.
Additional strain information