Strain information
NBRP Rat No: 0908
Strain name
: LE-Tg(Pdyn-cre)2-9Koba
Commmon Name: Pdyn_Cre RecBAC 2-9ラット
Rat Genome Database
Principal Investigator:
Kazuto Kobayashi Fukushima Medical University 1 Hikarigaoka Fukushima-shi 960-1295 Fukushima Japan
Tel: 024-547-1667 Fax: 024-548-3936
Email: kazuto@fmu.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白黒
Inbred Generations
F9 (April 2019)
Usage Restrictions
The following specific terms and conditions are requested by the DEPOSITOR. In this event, the RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Tha RECIPIENT requires a collabration with the DEPOSITOR. Consult with the DEPOSITOR about details beforehand.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others 神経科学一般
Control Strain
Marker Strain
Gene Affected
cre: cre recombinase protein, Pdyn: preprodynorphin
Origin
Background strain: Long-Evans (Iar:Long-Evans, Institute for Animal Reproduction) This strain was produced by injecting a modified BAC, in which a Cre recombinase was inserted into the third exon of the rat Pdyn gene, into rat zygotes.
Strain characteristics
This strain is a transgenic rat that expresses Cre recombinase under the control of the Pdyn gene.
Breeding Conditions
Breeding performance is good. Maintain heterozygous rats by mating them with Long Evans (LE) rats.
Genotyping
The PCR method is used to detect Cre recombinase. Primer F: TCGATGCAACGAGTGATGAG, R: TTCGGCTATACGTAACAGGG PCR conditions TaKaRa Taq 94℃ 5min→94℃(30cycle) 20sec→56℃ 30sec→72℃ 30sec→4℃ ∞ 482bp
References
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Submitted for publication.
Additional strain information